Hepatotoxicity of tubers of Indian Kudzu (Pueraria tuberosa) in rats |
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| Authors: | Nagwani Santosh Kumar Mohan Singh Royana Tripathi B. Yamini |
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| Affiliation: | 1. Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India;2. Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India;3. Department of Anatomy, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India |
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| Abstract: | Methanolic extract of tubers of Pueraria tuberosa Linn. (Fabaceae) (PTME) has been tested for hepatoxicity in rats. In acute study, PTME (100–400 mg/100 g BW, given orally) showed LD50 at 227.5 mg. For sub-chronic study, its repeated doses (5–100 mg/100 g BW, for 30 days), significantly increased hepatic enzymes in blood, sinusoidal congestion, disruption of central vein, inflammatory cell infiltration and hepatocellular necrosis in liver in dose dependent manner, with increase in NO, iNOS and ROS levels. In a kinetic study (single dose 227.5 mg/100 g BW), there was sequential decrease in GSH and enhanced NO suggesting free-radical generation as the primary cause of cell damage. It is concluded that the higher dosing of PTME or its continuous use for longer period (even in low doses) is hepatotoxicity by inducing oxidative stress. |
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| Keywords: | ALT, alanine aminotransferase AST, aspartate aminotransferase BHU, Banaras Hindu University BT, bilirubin total BD, bilirubin direct BUN, blood urea nitrogen BW, body weight CRE, creatinine CAT, catalase DTNB, 5,5-dithiobis-2-nitrobenzoic acid GLU, glucose GSH, reduced glutathione iNOS, inducible nitric oxide LPO, lipid peroxidation LD50, median lethal dose MDA, malondialdehyde NBT, nitro blue tetrazolium NO, nitric oxide PTME, methanolic extract of tubers of Pueraria tuberose Linn. PT tubers, powder of Pueraria tuberosa ROS, reactive oxygen species SOD, superoxide dismutase TBARS, thiobarbituric acid reacting substance TLC, thin layer chromatography TP, total protein TNF-α, tumor necrosis factor- α IL, interleukin IF, interferon |
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