Glycine reduces cadmium-induced alterations in the viability and activation of macrophage U937 cells |
| |
Authors: | Tebekeme Okoko Ejiro Prosper Awhin |
| |
Institution: | 1. Biochemistry Programme, Department of Chemical Sciences, Niger Delta University, PMB 71, Wilberforce Island, Bayelsa State, Nigeria;2. Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Delta State University, Abraka, Delta State, Nigeria |
| |
Abstract: | This study investigates the effect of glycine on cadmium-induced alterations on the viability and activation of the cell line U-937. In this experiment, U-937 cells were pre-treated with 16 μM cadmium (as cadmium chloride). These cadmium-treated cells were later incubated with or without glycine (1–16 μM). After 72 h, it was revealed that glycine significantly (P < 0.05) reduced the tendency of cadmium to reduce the viability of the cells. U-937 cells were also treated with phorbol, 12-myristate, 13-acetate to enhance their transition to the macrophage form. Thereafter, the cells were treated with cadmium with or without glycine (1–16 μM). Twenty-four hours later, the supernatants of each cell culture were assessed for the production of tumour necrosis factor alpha (TNF-α), interleukin 6 (IL-6), interleukin 1 (IL-1), nitric oxide (NO), and catalase activity as indices of the activation of macrophages. The results show that glycine significantly (P < 0.05) reduced the cadmium-induced production of all the markers of the activation of macrophages in a concentration-dependent manner. The findings support the immense antioxidant role of glycine. |
| |
Keywords: | Cadmium Glycine Macrophage Nitric oxide Antioxidant |
本文献已被 ScienceDirect 等数据库收录! |
|