In vitro screening of essential oil from young and mature leaves of Artemisia scoparia compared to its major constituents for free radical scavenging activity |
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Authors: | Harminder Pal Singh Shalinder Kaur Sunil Mittal Daizy R Batish Ravinder K Kohli |
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Institution: | 1. Department of Environment and Vocational Studies, Panjab University, Chandigarh 160 014, India;2. Department of Botany, Panjab University, Chandigarh 160 014, India |
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Abstract: | The present study investigated the chemical characterization, and antioxidant activity of essential oil hydrodistilled from young and mature leaves of Artemisia scoparia. GC–MS analyses revealed a monoterpenoid nature (64–67%) with 44 and 31 constituents in young and mature leaves oil, respectively. The oil from young leaf contained greater amount of oxygenated compounds. β-Myrcene (24.13%) and p-cymene (27.06%) were the major constituents in young and mature leaves oil, respectively. A. scoparia leaf oils (25–200 μg/ml) exhibited a strong 2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity and antioxidant activity against hydroxyl radical and hydrogen peroxide. However, the activities of major constituent monoterpenes, β-myrcene and p-cymene, were less. In general, the DPPH radical scavenging and antioxidant activity was in the order: mature leaf oil > young leaf oil > β-myrcene > p-cymene. |
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Keywords: | Artemisia scoparia (redstem wormwood) Leaf essential oil GC&ndash MS analyses Monoterpenoids DPPH scavenging activity Antioxidant activity |
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