Measuring behavior in mice with chronic stress depression paradigm |
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Authors: | Tatyana Strekalova Harry WM Steinbusch |
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Institution: | 1. Department of Neuroscience, Faculty of Health, Medicine and Life Sciences, Maastricht University, Universiteitssingel 50, NL 6229 ER Maastricht, Netherlands;2. Center of Environmental Biology, Lisbon University, Faculty of Sciences, Campo Grande, 1749-016 Lisbon, Portugal |
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Abstract: | Many studies with chronic stress, a common depression paradigm, lead to inconsistent behavioral results. We are introducing a new model of stress-induced anhedonia, which provides more reproducible induction and behavioral measuring of depressive-like phenotype in mice. First, a 4-week stress procedure induces anhedonia, defined by decreased sucrose preference, in the majority of but not all C57BL/6 mice. The remaining 30–50% non-anhedonic animals are used as an internal control for stress effects that are unrelated to anhedonia. Next, a modified sucrose test enables the detection of inter-individual differences in mice. Moreover, testing under dimmed lighting precludes behavioral artifacts caused by hyperlocomotion, a major confounding factor in stressed mice. Finally, moderation of the stress load increases the reproducibility of anhedonia induction, which otherwise is difficult to provide because of inter-batch variability in laboratory mice. We believe that our new mouse model overcomes some major difficulties in measuring behavior with chronic stress depression models. |
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Keywords: | CMS chronic mild stress DSM Diagnostic and Statistical Manual |
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