Inhibitory effects of quercetin on aflatoxin B1-induced hepatic damage in mice |
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| Authors: | K-C Choi W-T Chung J-K Kwon J-Y Yu Y-S Jang S-M Park S-Y Lee J-C Lee |
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| Institution: | 1. National Livestock Research Institute, RDA, Suweon 441-706, South Korea;2. Foundation of Agri. Tech. Commercialization & Transfer, Suweon 441-706, South Korea;3. Department of Laboratory Animal Medicine, College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, South Korea;4. Division of Biological Sciences, Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 561-756, South Korea;5. Institute of Oral Biosciences (BK 21 Program), Research Center of Bioactive Materials, Chonbuk National University, Jeonju 561-756, South Korea;6. Division of Biotechnology, Chonbuk National University, Iksan 570-752, South Korea;g Department of Dentistry, Gangneung-Wonju National University, Gangwon 210-702, South Korea |
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| Abstract: | Aflatoxin B1 (AFB1)-mediated hepatic damage is involved in production of AFB1-8,9-epoxide-bound DNA adducts and this is also affected by a pro-oxidant potential of the toxin. In this study we investigated the effects of quercetin on AFB1-treated HepG2 cells. We also examined the biochemical mechanisms associated with the effects of quercetin on AFB1-mediated liver damage in mice. Our results revealed that quercetin and isorhamnetin inhibit production of reactive oxygen species and cytotoxicity, and block the decrease of reduced glutathione (GSH) levels in AFB1-treated HepG2 cells. Isorhamnetin have inhibitory ability on lipid peroxidation stronger than quercetin in the cells. Oral supplementation with quercetin decreased serum lactate dehydrogenase levels, increased hepatic GSH levels and superoxide dismutase activity, and reduced lipid peroxidation in both the liver and kidney in AFB1-treated mice. However, quercetin did not show a significant reduction on serum levels of alkaline phosphate, alanine aminotransferase and aspartate aminotransferase that were increased in AFB1-treated mice. HPLC analysis revealed that quercetin in plasma is mainly present as glucoronides and/or sulfates of quercetin. Collectively, it is suggested that quercetin does not directly protect against AFB1-mediated liver damage in vivo, but exerts a partial role in promoting antioxidative defense systems and inhibiting lipid peroxidation. |
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| Keywords: | Aflatoxin Quercetin Liver Lipid peroxidation Antioxidative defense enzymes |
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