Antioxidant and antimicrobial properties of Moltkia petraea (Tratt.) Griseb. flower,leaf and stem infusions |
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Authors: | M. Zovko Končić,D. Kremer,J. Gruz,M. Strnad,G. Bi&scaron evac,I. Kosalec,D. &Scaron amec,J. Piljac-Žegarac,K. Karlović |
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Affiliation: | 1. Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kova?i?a 1, 10000 Zagreb, Croatia;2. Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany ASCR, Šlechtitel? 11, CZ-783 71 Olomouc, Czech Republic;3. Department of Molecular Biology, Institute ‘Ru?er Boškovi?’, Bijeni?ka c. 54, P.O. Box 180, 10000 Zagreb, Croatia;4. Faculty of Agriculture, University of Zagreb, Svetošimunska 25, 10000 Zagreb, Croatia |
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Abstract: | Antioxidant and antimicrobial activities as well as the quantity of phenolic substances (total phenol, flavonoid and phenolic acid contents) were determined in aqueous extracts of leaves, stems and flowers of Moltkia petraea (Tratt.) Griseb. from two mountainous localities (Sveti Jure and Snije?nica) in Croatia. In addition, the profile of phenolic acids was analyzed by UPLC–MS/MS. Antioxidant activities of all extracts in different test systems, namely the DPPH radical scavenging, reducing power assay and chelating activity, increased with extract concentration. Activity of the extracts from Snije?nica in β-carotene-linoleic acid assay did not differ from the activity of standard, BHA. The leaf extracts from Snije?nica demonstrated superior antioxidant activity in most of the assays, while the stem extract from the same locality was the most effective Fe2+ ion chelator. In general, the extracts from Snije?nica were more effective antioxidants than the corresponding extracts from Sveti Jure. The aqueous extracts of M. petraea did not show antimicrobial activity against bacteria and fungi tested in the diffusion and dilution assays. |
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Keywords: | ASC, ascorbic acid BHA, butylated hydroxyanisol ChA, chelating activity DPPH, 2,2-diphenyl-1-picrylhydrazyl ROS, reactive oxygen species RSA, free radical scavenging activity TF, total flavonoid content TP, total phenol content TPA, total phenolic acid content UPLC, ultra performance liquid chromatography |
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