Delphinidin-3-O-glucoside,an active compound of Hibiscus sabdariffa calyces,inhibits oxidative stress and inflammation in rabbits with atherosclerosis |
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Authors: | Bo Sun Fangda Li Xu Zhang Wei Wang Jiang Shao Yuehong Zheng |
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Affiliation: | aDepartment of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China;bDepartment of Vascular Surgery, Weifang People’s Hospital, Weifang, China |
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Abstract: | ContextDelphinidin-3-O-glucoside (DP) is a bioactive compound of Hibiscus sabdariffa L. (Malvaceae) (Roselle) calyces and exerts endothelial protection and lipid-lowering activities, which provided a basis for the prevention and treatment of cardiovascular diseases.ObjectivesTo investigate the therapeutic effects of DP against atherosclerosis.Materials and methodsA rabbit model of atherosclerosis (AS) was established by 12 weeks of a high-fat diet (HFD). The rabbits were divided into five groups: control, AS, simvastatin (4 mg/kg), and two DP groups (10 and 20 mg/kg). After treatment with DP or simvastatin by oral gavage for 12 weeks, the lipid profiles were measured. Histopathological assessment of the aorta was performed by H&E staining. Oxidative stress and inflammation-related markers were analyzed by ELISA kit and real-time RT-PCR.ResultsDP (20 mg/kg) decreased serum TG (2.36 ± 0.66 vs. 4.33 ± 0.27 mmol/L for the AS group), TC, LDL-C, and HDL-C (all p < 0.05). DP (20 mg/kg) also reduced lipid levels in the liver and aorta. DP (20 mg/kg) down-regulated the mRNA levels of IL-6, VCAM-1, and NF-κB and up-regulated the mRNA levels of GSH-PX and SOD1.ConclusionsThis study proved that DP alleviated the HFD-induced oxidative stress and inflammation in atherosclerosis rabbits. These results provided the scientific basis for developing novel therapies. |
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Keywords: | Hypolipidemic effect antioxidant effect inflammatory cytokines high-fat diet |
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