Heat-shock treatment in a rapid assay for cytomegalovirus detection in clinical samples |
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| Affiliation: | 1. National Centre for Neuroimmunology and Emerging Diseases (NCNED), Griffith University, Gold Coast, QLD, Australia;2. Centre for Advanced Imaging, The University of Queensland, St. Lucia, Australia |
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| Abstract: | A mild heat-shock treatment of cells enhances cytomegalovirus (CMV) infection and shortens its eclipse period. This provided the basis for the development of a rapid assay to detect CMV in clinical samples.Urine specimens were inoculated in heat-shocked cells and then CMV-infected cells were visualized at various hours after infection with a monoclonal antibody directed against a CMV-induced immediate-early protein, using an immunoalkaline phosphatase staining. Out of 104 urine specimens examined, 13 proved positive and the assay we describe was able to yield positive results in a single day. |
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