饮食诱导小鼠肥胖疾病模型的建立

建立适合的食源性肥胖症动物疾病模型,用于评价减肥药物临床前动物实验的有效性和安全性。方法将120只C57BL/6J小鼠按随机数字表法分为2组,模型组(n=110)采用高脂饲养饮食诱导肥胖(DIO),对照组(n=10)采用标准饲料饲养,2组均饲养12周。每周监测小鼠体质量和摄食量的变化,ELISA法检测血浆中血糖、血脂和胰岛素水平,断尾采血进行糖耐量(OGTT)试验。结果饲养12周后,模型组和对照组小鼠体质量分别为(36.90±5.07)、(27.68±2.27)g,2组比较差异有统计学意义(P<0.001);模型组有80.9%的小鼠发育为DIO;模型组较对照组摄食量明显增加(P<0.01)。与对照-OGTT组相比,DIO-OGTT组血糖和胰岛素明显增加(P<0.01),并伴有糖耐量减低和高脂血症。结论饮食诱导可成功建立小鼠肥胖疾病模型,高脂饮食可以导致具有肥胖倾向的小鼠体质量增加,此模型与临床肥胖症病理接近,且具有稳定性好、操作简单、费用低等优点。更多还原

Establishment of A Mouse Model of Diet-induced Obesity

Objective To establish an animal model of diet-induced obesity(DIO),and to investigate the efficacy and safety of weight-loss drugs in pre-clinical animal testing.Methods A total of 120 C57BL/6J mice were randomly divided into fed high-fat diet(Research Diets Inc.,D12492,60%kcal% fat;DIO group,n=110) or standard chow(control group,n=10) for 12 weeks.Food intake and body weight were measured weekly.The levels of plasma glucose,insulin and lipid were detected by enzyme-linked immunosorbent assay(ELISA).Blood samples were collected by tail cutting for glucose tolerance test.Results After feeding for 12 weeks,80.9% of mice in DIO group developed obesity and had impaired glucose tolerance,and hyperlipidemia.Compared with control group,body weight was significantly increased in DIO group [(36.90±5.07)g vs(27.68±2.27)g,P<0.001].Moreover,food intake and levels of plasma glucose and insulin obviously elevated compared with control group(P<0.01).Conclusion High-fat diet feeding can induce DIO and increase body weight in mice.The established model is closed with clinical obesity with the advantages of good stability,simple operation and low cost.