阿托伐他汀对心肌梗死大鼠心肌纤维化和细胞外信号调节激酶表达的影响

目的观察阿托伐他汀对大鼠急性心肌梗死后心室重塑和心肌组织中细胞外信号调节激酶(ERK)1/2蛋白表达的影响。方法 20只雄性SD大鼠通过前降支动脉结扎法建立大鼠急性心肌梗死模型后随机分为心肌梗死组(AM I),心肌梗死阿托伐他汀组(ATV),另设假手术组(sham),每组n=10。阿托伐他汀组每天给予阿托伐他汀(10 mg/kg)灌胃,持续4周。假手术组和心肌梗死组每天给予同等量的0.9%氯化钠注射溶液灌胃。应用real-tim e PCR法检测梗死周边区心肌组织中Ⅰ型、Ⅲ型胶原mRNA表达,应用westernb lot方法检测p-ERK1/2、ERK1/2蛋白的表达。结果与假手术组比较,心肌梗死组和阿托伐他汀组中Ⅰ型、Ⅲ型胶原表达高于假手术组(P<0.01),阿托伐他汀组Ⅰ型、Ⅲ型胶原表达显著低于心肌梗死组(P<0.01)。心肌梗死组和阿托伐他汀组中p-ERK1/2表达高于假手术组(P<0.01),阿托伐他汀组p-ERK1/2表达低于心肌梗死组(P<0.01)。结论阿托伐他汀可以改善大鼠心肌梗死后心室重塑,其机制可能与下调心肌组织中p-ERK1/2表达有关。

The effects of atorvastatin on myocardial fibrosis and expression of extracellular signal-regulated kinase 1/2in rats after acute myocardial infarction

Objective To investigate the effects of atorvastatin on myocardial fibrosis and expression of extracellular signal-regulated kinase 1/2（ERK1/2）in rats after acute myocardial infarction.Methods 20 male SD rats were induced by left anterior descending artery ligation,Then they were randomly divided into AMI group and AMI ＋ atorvastatin group.10 male SD rats were sham-operated.Rats in atorvastatin group were treated with atorvastatin gavage（10mg/Kg/day）every day for 4 weeks.In AMI group and sham-operated group rats were treated with drinking water.CollagenⅠand collagen Ⅲ in myocardial tissue were detected by real-time PCR.p-ERK1/2,ERK1/2 in myocardial were measured by western blot.Results Compared with sham-operated group,collagenⅠand collagen Ⅲ in other groups were significantly increased（P0.01）.However,atorvastatin decreased the expression of collagenⅠand collagen Ⅲ（P0.01）.The expression of p-ERK1/2 in other groups were significantly increased（P0.01）,However,atorvastatin decreased the expression of p-ERK1/2（P0.01）.Conclusion Atorvastatin may ameliorated left ventricular remodeling after MI in rats,and decreased myocardial p-ERK1/2 expression.