Evidence for tyrosine at the ligand binding center of beta-adrenergic receptors |
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| Authors: | R G Shorr M D Minnich L Gotlib A Varrichio M W Strohsacker S T Crooke |
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| Institution: | 1. Academy for Educational Development, 1825 Connecticut Ave., NW, Washington, DC 20009, USA;2. Department of International Health, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA;3. Jhpiego, 1615 Thames Street, Baltimore, MD 21231-3492, USA;4. Shimantik, 581 Shewrapara, Mirpur, Dhaka 1216, Bangladesh;1. Texas Tech University Health Sciences Center, Paul L. Foster School of Medicine, El Paso, Texas;2. Texas Tech University Health Sciences Center, Department of Dermatology, Lubbock, Texas;3. Cosmetic Laser Dermatology, San Diego, California |
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| Abstract: | Beta 1- and beta 2-adrenergic receptor radioligand antagonist binding activities in plasma membrane preparations from mammalian lung, as well as amphibian and avian red blood cells, have been shown to be inactivated by agents which specifically alkylate tyrosine. In membrane preparations, protection against inactivation was afforded by both agonists and antagonists. In soluble purified preparations, antagonists but not agonists protected against inactivation. These results suggest that tyrosine is located at or near the ligand binding site of both beta 1- and beta 2-adrenergic receptors. |
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