Serotonin induces EPSCs preferentially in layer V pyramidal neurons of the frontal cortex in the rat |
| |
Authors: | Lambe E K Goldman-Rakic P S Aghajanian G K |
| |
Affiliation: | Interdepartmental Neuroscience Program, Section of Neurobiology, and Departments of Psychiatry and Pharmacology, Yale University School of Medicine, New Haven, CT, USA. evelyn.lambe@yale.edu |
| |
Abstract: | The effect of serotonin (5-HT) on the release of glutamate was examined in pyramidal cells in layers II-VI of the frontal cortex. The intracellular recording electrode contained 1% biocytin so the neurons could later be visualized with an avidin-biotin peroxidase method. Pyramidal cells in layer V of the frontal cortex showed the greatest 5-HT-induced increase in both the frequency and amplitude of 'spontaneous' (non-electrically evoked) excitatory post-synaptic currents (EPSCs). A small proportion of neurons in layer II/III showed an increase in EPSC frequency, whereas cells in layer VI showed no significant change in either EPSC frequency or amplitude. The physiological response to 5-HT mirrors the high density of 5-HT(2A) receptors in layer V, as well as the pattern of thalamic projections in frontal cortex. The specific induction of EPSCs in layer V neurons suggests that 5-HT preferentially modulates the output neurons of the frontal cortex. |
| |
Keywords: | |
本文献已被 PubMed Oxford 等数据库收录! |
|