蛋白激酶C在非心脏器官预处理中的作用

目的探讨蛋白激酶Ｃ（ＰＫＣ）在心脏以外组织器官缺血或缺氧预处理（ＰＣ）保护中的作用及其机理。方法分别在原位灌流的大鼠小肠、肢体缺血再灌注模型和培养的大鼠胸主动脉血管平滑肌细胞（ＶＳＭＣ）缺氧复氧模型上观察ＰＫＣ对于器官缺血或细胞缺氧ＰＣ作用的影响。结果ＰＫＣ抑制剂Ｈ７和多粘菌素Ｂ完全消除缺血ＰＣ对小肠和肢体的保护作用;缺氧ＰＣ激活ＶＳＭＣ内ＰＫＣ,并使ＰＫＣ介导的蛋白磷酸化反应加强。结论ＰＫＣ激活是心脏以外组织器官ＰＣ保护的重要环节,其机制可能与底物蛋白的磷酸化有关。

Effects of protein kinase C on non-heart organ protection of preconditioning

Objective To investigate the effects of protein kinase C (PKC) on ischemic (hypoxic) preconditioning (PC) in the non heart organs and tissue. Methods In the models of ischemia/reperfusion of in situ perfused rat small intestine, limb, and in the model of hypoxia/ reoxygenation of cultured rat aortic vascular smooth muscle cells (VSMC), the effects of PKC on ischemic (hypoxic) PC were observed. Results PKC inhibitor H7 and polymyxin B completely abolished the protective effects of ischemic PC on small intestine and limb subjected to ischemia/ reperfusion injury. Hypoxic PC activated PKC in VSMC and potentiated phosphorylation mediated by PKC. Conclusion The activation of PKC is also the important chain of PC on non heart organs, and its mechanism might involve protein phosphorylation.