| 新霉素对脑胶质瘤细胞增殖及PDGF、VEGF和血管生成素表达的影响 |
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| 引用本文： | 赵佳,郝利铭,姜文华,孔德霞,李洪成,周莉.新霉素对脑胶质瘤细胞增殖及PDGF、VEGF和血管生成素表达的影响[J].中国实验诊断学,2014(10):1579-1582. |
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| 作者姓名： | 赵佳 郝利铭 姜文华 孔德霞 李洪成 周莉 |
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| 作者单位： | 吉林大学基础医学院 组织学与胚胎学系,吉林 长春,130021 |
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| 摘 要： | 目的：探讨新霉素对脑胶质瘤细胞增殖及PDGF、VEGF和血管生成素表达的影响。方法采用人脑胶质瘤细胞U251，DMEM培养基添加10％胎牛血清培养。MTT细胞活性实验检测细胞增殖，RealTimePCR检测mR-NA表达情况，酶联免疫吸附实验检测蛋白表达。结果MTT结果表明，新霉素对U251细胞增殖存在抑制作用，并以剂量依赖的方式进行，并发现新霉素的抑制作用随时间增强。RealTimePCR结果显示，新霉素作用后U251细胞中PDGF、VEGF和血管生成素mRNA的表达都受到不同程度的抑制，分别降低了26．75％、38．23％和43．87％。ELISA分析表明新霉素能够降低PDGF、VEGF和血管生成素蛋白水平的表达。结论新霉素能够抑制脑胶质瘤细胞U251中PDGF、VEGF和血管生成素的表达并抑制脑胶质瘤细胞增殖。
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| 关 键 词： | 新霉素 脑胶质瘤 PDGF VEGF 血管生成素 |
Effect of neomycin on cell proliferation and expression of PDGF,VEGF,angiogenin in glioma cells |
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| Institution: | ZHAO Jia , HAO Li- ming , JIANG Wen-hua ,et al. (Department of Histology and Embryology, College of Basic Medical Sciences, Jilin University ,Changchun 130021 ,China) |
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| Abstract: | Objective To observe the effect of neomycin on cell proliferation and expression of PDGF,VEGF,angio-genin in U251 glioma cells.Methods The cell proliferation was analyzed using MTT.Real Time PCR and ELISA were applied to investigate the expression of PDGF,VEGF,angiogenin.Results The MTT results showed that neomycin positively inhibited the cell proliferation of U251 cells and the inhibition was enhanced by dose-dependent and time-de-pendent.Real Time PCR and ELISA results showed that neomycin inhibited the expression of PDGF,VEGF,angiogenin both on mRNA level and protein level.Conclusion Neomycin positively inhibited the cell proliferation and expression of PDGF,VEGF,angiogenin in U251 glioma cells. |
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| Keywords: | neomycin glioma PDGF VEGF angiogenin |
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