| 人乳腺珠蛋白基因真核表达载体的构建及其表达 |
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| 引用本文： | 赵矫,田强,赵小平,税青林,余红,刘岚.人乳腺珠蛋白基因真核表达载体的构建及其表达[J].现代预防医学,2010,37(1). |
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| 作者姓名： | 赵矫 田强 赵小平 税青林 余红 刘岚 |
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| 作者单位： | 泸州医学院生物学与遗传学教研室,泸州,646000 |
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| 基金项目： | 四川省卫生厅科学研究项目，四川省泸州医学院科学研究项目 |
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| 摘 要： | 目的]构建人乳腺珠蛋白(hMAM)基因的真核表达载体pcDNA3.1-hMAM,并观察重组载体在COS-7细胞中的表达,为肿瘤DNA疫苗研究奠定基础.方法]利用PCR方法扩增hMAM基因,酶切测序分析后,克隆入真核表达载体pcDNA3.1,构建真核表达载体pcDNA3.1-hMAM.将重组载体用脂质体转染法转染入COS-7细胞,间接免疫荧光法检测目的基因的表达.结果]成功扩增hMAM基因,酶切测序结果表明,重组载体含有hMAM基因,在COS-7细胞中可检测到hMAM表达.结论]hMAM基因的真核表达载体构建成功,为进一步进行DNA肿瘤疫苗研究提供了实验依据.
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| 关 键 词： | 人乳腺珠蛋白 COS-7细胞 重组质粒 |
STUDY ON CONSTRUCTION AND EXPRESSION OF EUKARYOTIC EXPRESSION PLASMID OF HUMAN MAMMOGLOBIN (HMAM) GENE |
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| ZHAO Jiao,TIAN Qiang,ZHAO Xiao-ping,et al..STUDY ON CONSTRUCTION AND EXPRESSION OF EUKARYOTIC EXPRESSION PLASMID OF HUMAN MAMMOGLOBIN (HMAM) GENE[J].Modern Preventive Medicine,2010,37(1). |
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| Authors: | ZHAO Jiao TIAN Qiang ZHAO Xiao-ping |
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| Institution: | ZHAO Jiao,TIAN Qiang,ZHAO Xiao-ping,et al. (Luzhou Medical College,Luzhou 646000,China) |
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| Abstract: | Objective] To construct the eukaryotic expression plasmid of hMAM gene and identify its expression in COS-7 cells,in order to lay a foundation for further development of DNA vaccine against breast cancer. Methods] The hMAM and gene was amplified by PCR. After enzyme digestion and DNA sequencing,the gene was cloned into the eukaryotic expression vector pcDNA3.1 to construct recombinant plasmid pcDNA3.1-hMAM. Then the recombinant plasmid was transfected into COS-7 cells by LipofectamineTM 2000 and the expre... |
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| Keywords: | hMAM COS-7 cell Recombinant plasmid |
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