胃癌中CD44V6与p53蛋白的表达及其意义

 目的　分析胃癌研究中的两个热点基因蛋白CD44V6和p53与胃癌发生、发展及转移的关系。方法　运用免疫组化法，检测78例原发胃癌及癌旁良性病变的CD44V6及p53基因蛋白的表达。结果　30例胃癌出现CD44V6的阳性表达38.5%(30/78)，癌旁良性病变中未检测到其表达；侵及浆膜或具有淋巴结转移及远距离转移者，V6表达率明显高于无浆膜受侵或无淋巴结转移及远距离转移者。肠型胃癌的V6表达率明显高于弥漫型胃癌的，但弥漫型胃癌中V6表达与淋巴结转移呈显著相关性，而肠型胃癌中则否。有28例胃癌检测到p53蛋白的积聚35.9%(28/78)，p53阳性与各种临床病理因素均无关，但癌旁良性病变中却可见p53阳性。结论　CD44V6表达预示胃癌具有较强的侵袭转移能力，而p53基因蛋白的异常表达与细胞的早期癌变有关。

Siginificance and Expression of CD44V6 and p53 Genes in Gasrtric Carcinoma

Objective To study two topic genes in gastric carcinoma-CD44 and p53,and the relationship between they and carcinogensis,invasion and metastasis of gastric carcinoma.Methods Expression of CD44 and p53 genes was immunohistochemically detected in 78 gastric carcinomas and 46 benign gastric mucosa lesions adjacent to carcinoma by monoclonal antibodies of 2F10 (anti-CD44V6) and DO-1 (anti-p53).The relationship between CD44 and p53 gene expression and each of the clinicopatholgic characteristics was analysed and statistically tested.Results Expression of CD44V6 was observed in 30 gastric carcinornas (38.5%),not in benign mucosa lesions adjacent to carcinoma.The incidence of CD44V6 positivity was higher in carcinomas with serosa invasion,lymph node or distance metastasis than those without (P<0.05).The intestinal type carcinoma was more frequently CD44V6 positive than the diffuse type carcinoma (P<0.05).However,expression of CD44V6 was significantly associated with node metastasis in the diffuse type (P<0.05)，not in the intestinal type carinoma.CD44V6 positivity had no relation to tumor size,age and sex of patients.p53 protein accumulation was detected in 28 gastric carcinomas (35.9%) and 5 benign lesions adjacent to carcinoma (10.9%),prositivity of p53 protein was not correlated with each of the clincopathologic characteristics.Conclusion These results suggested that CD44V6 may be involved in invasion and metastasis of gastric carcinoma.Its expression indicated that gastric carcinoma had stronger invasion and metastasis potential.p53 gene alteration occurred in early stage of gastric carcinoma development ,and may be associated with gastric epithelium carcinogenesis.