Structure of the cloned Locusta migratoria mitochondrial genome: restriction mapping and sequence of its ND-1 (URF-1) gene |
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Authors: | A. McCracken I. Uhlenbusch G. Gellissen |
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Affiliation: | (1) Department of Biology, Queen's University, K7L 3N6 Kingston, Ontario, Canada;(2) Institut für Zoologie III, Universität Dusseldorf, Universitätsstrasse 1, D-4000 Düsseldorf 1, Germany |
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Abstract: | Summary We have cloned the entire mitochondrial genome of Locusta migratoria in four fragments and characterised by restriction mapping. In addition, we have sequenced a 1,095 kb region containing the ND-1 (URF-1) gene. The inferred primary structure of the protein is highly homologous to its Drosophila counterpart (68%). The gene is flanked at the 5 end by the tRNACUNleugene, interrupted by the sequence TTG. The 3 end is flanked by the tRNAserUCNgene, followed by a sequence homologous to the 3 end of D. yakuba cytochrome b. The relative position of the genes is conserved between Locusta and Drosophila, thus indicating conservation of mitochondrial gene order in insects. |
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Keywords: | Locusta migratoria Mitochondrial genome ND-1 |
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