广东凉茶颗粒对拘束负荷诱发小鼠应激性肝损伤的保护作用

目的:研究广东凉茶颗粒对拘束负荷诱发小鼠应激性肝损伤的保护作用。方法:将实验小鼠随机分成正常对照组、拘束模型组、维生素C 250 mg.kg^-1给药组、广东凉茶颗粒500,250 mg.kg^-1给药组。18 h拘束负荷后诱发小鼠应激性肝损伤,分别用赖氏法测定小鼠血浆丙氨酸氨基转移酶(ALT)活性,硫代巴比妥酸法测定肝组织和血浆丙二醛(MDA)水平,HPLC法测定谷胱甘肽(GSH)含量,比色法测定谷胱甘肽过氧化物酶(GSH-PX)活性、谷胱甘肽S转移酶(GST)活性,G riess化学法测定一氧化氮(NO)水平和荧光酶标仪测定抗氧化能力指数(ORAC)。结果:与拘束负荷模型组相比,广东凉茶颗粒可以明显降低因拘束负荷引起的小鼠血浆ALT活性(92.75±1.91 vs 39.29±2.56,32.69±1.46)U.L^-1,保护拘束负荷诱发的应激性肝损伤。此外,广东凉茶颗粒有效地提高应激小鼠肝组织中ORAC指数、GSH含量、GSH-PX及GST活性,降低MDA和NO水平。结论:广东凉茶颗粒对拘束负荷诱发小鼠应激性肝损伤具有一定的保护作用,其作用机制可能部分来自于减少拘束负荷小鼠氧化应激水平和改善组织脂质过氧化过程。

Protective effects of Guangdong Liangcha grandes on restraint stress-induced liver damage in mice

Objective: To investigate the effect of Guangdong Liangcha Keli on restraint stress-induced liver damage in mice.Method: Thirty-five male C57BL/6J mice of 7 weeks old were divided into 5 groups randomly with 7 mice in each group: normal group,restraint stress group,250 mg·kg^-1 Vitamin C,Guangdong Liangcha Keli 500 mg·kg^-1 and 250 mg·kg^-1.After 18 hr restraint stress,the ALT acitivity in plasma,MDA level in plasma and liver,GSH content,GSH-PX and GST activities,NO level and ORAC value in liver were determined.Result: Compared with restraint model group,Guangdong Liangcha Keli could markedly reduce ALT activity(92.75±1.91 vs 39.29±2.56,32.69±1.46) U·L^-1,and protect the liver damage induced by oxidative stress.In addition,Guangdong Liangcha Keli could effectively increase the ORAC value,GSH content,GSH-PX activity and GST activity and reduce the MDA level and NO level in liver.Conclusion: Oral treatment of Guangdong Liangcha Keli is found to reduce restraint stress-induced liver damage in terms of above mentioned biochemical parameters,and these protective effects may be related to its free radical scavenging activity and lipid peroxidation inhibitory effects.