七氟烷对成年大鼠认知功能影响的研究

目的探讨不同浓度（1%，2%，3%）七氟烷对成年大鼠的认知功能及血清、海马组织中神经元特异性烯醇化酶（NSE）的影响。方法将40只成年雄性SD大鼠随机分为1%七氟烷组、2%七氟烷组、3%七氟烷组及对照组（吸入空气），七氟烷吸入3h。处理后第1，2，3，4，5天进行Morris水迷宫的定位航行试验和第6天的空间探索试验。第6天测试完毕后取大鼠的血清和海马组织测定NSE的含量。结果吸入不同浓度七氟烷组与对照组相同时间点比较，逃避潜伏期和总路程差异无统计学意义（P＞0.05）。同时，不同浓度七氟烷组间比较，逃避潜伏期和总路程差异也无统计学意义（P＞0.05）。吸入七氟烷或空气后第6天撤台，各组大鼠的目标象限停留时间百分比和穿越平台次数之间差异也无统计学意义（P＞0.05）。吸入1%和3%七氟烷组大鼠血清中NSE水平低于对照组（P＜0.01）；同时，吸入2%和3%七氟烷组大鼠血清中NSE水平高于吸入1%七氟烷组（P＜0.01，P＜0.05）。海马组织中的NSE含量各组间差异无统计学意义（P＞0.05）。结论单次吸入1%，2%和3%的七氟烷，短期不影响成年大鼠的空间学习记忆能力。同时，吸入1%和3%七氟烷可以降低大鼠血清中NSE水平。

Effects of sevoflurane on cognitive dysfunction in adult rats

Objective To determine the effect of sevoflurane at different doses （1%, 2%, 3%） on cognitive function and contents of neuron specific enolase （NSE） in the serum and hippocampus in adult rats. Methods Forty male adult Sprague Dawley（SD） rats were randomly divided into the following three treatment groups and control. The rats from the three treatment groups were exposed to 1%, 2% and 3% sevoflurane for 3h, respectively, while those of control were exposed to air. The navigation tests in Morris water maze （MWM） were performed on day 1, 2, 3, 4 and 5 after the treatment, and the pilot tests in MWM were performed on day 6. Blood samples and hippocampal tissues were collected from the rats in each group after the pilot tests on day 6 after the measurement for the content of NSE by enzyme linked immunosorbent assay. Results No significant difference was found in the escape latency and path length between the sevoflurane groups and control （P 〉 0.05）, and among sevoflurane groups of different doses （P 〉 0.05）. There was no significant difference in the mean percentage of crossing platform quadrant and the frequency of crossing platform among the four groups （P 〉 0.05）. The serum level of NSE was significantly lower in the 1% and 3% sevoflurane groups than in the control （P 〈 0.01）, and it was obviously higher in the 2% and 3% sevoflurane groups than in the 1% sevoflurane group （P〈 0.05 or P〈 0.01）. No significant difference was seen in the hippocampal content of NSE in the rats from the four groups （P 〉 0.05）. Conclusion The single exposure of sevoflurane at different doses has no short-term impact on the learning and memory in adult rats. The serum NSE level is decreased after exposure to 1% and 3% sevoflurane.