| 不同剂量酵母和腺嘌呤诱导大鼠痛风性肾病模型的建立比较 |
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| 引用本文： | 蓝伦礼,江滨,刘梦楚,邹晓红,曹骋,曾元儿,庞嘉颖.不同剂量酵母和腺嘌呤诱导大鼠痛风性肾病模型的建立比较[J].中国比较医学杂志,2017,27(2):33-43. |
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| 作者姓名： | 蓝伦礼 江滨 刘梦楚 邹晓红 曹骋 曾元儿 庞嘉颖 |
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| 作者单位： | 广州中医药大学中药学院中药分析实验室, 广州 510006;广州中医药大学中药学院中药分析实验室, 广州 510006;广州中医药大学中药学院中药分析实验室, 广州 510006;广州中医药大学中药学院中药分析实验室, 广州 510006;广州中医药大学中药学院中药分析实验室, 广州 510006;广州中医药大学中药学院中药分析实验室, 广州 510006;广州中医药大学中药学院中药分析实验室, 广州 510006 |
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| 基金项目： | 南药研究协同创新中心专项基金（2011）。 |
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| 摘 要： | 目的探讨不同剂量酵母、腺嘌呤和不同造模持续时间对大鼠痛风性肾病模型的影响。方法模型组雄性SD大鼠用15 g/kg酵母粉和50 mg/kg、100 mg/kg腺嘌呤分别灌胃,同时分别设置8、10、22、48的造模天数。比较各组肾脏肥大指数的大小,检测大鼠肾功能,尿液指标的变化,肾皮质β-N-乙酰氨基葡萄糖苷酶(NAG)含量,肝脏黄嘌呤氧化酶(XOD)活性的变化,同时观察大鼠肾脏病理改变。并用免疫组化技术检测肾脏组织NGAL、OAT1和TIMP-1的表达。结果随着模型组造模剂浓度的增加和造模时间的延长,肾脏病理改变越明显。100mg/kg腺嘌呤和15g/kg酵母共同造模8 d,与正常对照组比较,该组肾脏病理组织变化明显,炎性细胞浸润肾小管上皮细胞肿胀,肾小管和肾小球结构未被破坏;肾脏肥大指数较正常对照组增大(P0.01);与正常对照组比较,模型A组,B组、E组、F组肾功能下降(P0.01),模型A组、B组、E组、F组大鼠的24 h尿量,尿蛋白含量增多(P0.01)。模型E组大鼠肾组织NGAL、TIMP-1的表达高于正常组大鼠(P0.01),OAT1的表达低于正常组大鼠(P0.01)。结论 100 mg/kg腺嘌呤和15 g/kg酵母共同造模8 d为较理想的造模剂量和造模持续时间,痛风性肾病模型大鼠肾损伤受蛋白NGAL、TIMP-1和OAT1的影响。
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| 关 键 词： | 痛风性肾病 酵母 腺嘌呤 肾功能 |
| 修稿时间： | 2016/11/16 0:00:00 |
Comparison of the establishment of rat model of gouty nephropathy induced by yeast and adenine |
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| LAN Lun-li,JIANG Bin,LIU Meng-chu,ZOU Xiao-hong,CAO Chen,ZENG Yuan-er and Pang Jia-ying.Comparison of the establishment of rat model of gouty nephropathy induced by yeast and adenine[J].Chinese Journal of Comparative Medicine,2017,27(2):33-43. |
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| Authors: | LAN Lun-li JIANG Bin LIU Meng-chu ZOU Xiao-hong CAO Chen ZENG Yuan-er and Pang Jia-ying |
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| Institution: | Laboratory of Traditional Chinese Medicine Analysis, College of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Laboratory of Traditional Chinese Medicine Analysis, College of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Laboratory of Traditional Chinese Medicine Analysis, College of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Laboratory of Traditional Chinese Medicine Analysis, College of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Laboratory of Traditional Chinese Medicine Analysis, College of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Laboratory of Traditional Chinese Medicine Analysis, College of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;Laboratory of Traditional Chinese Medicine Analysis, College of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China |
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| Abstract: | |
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| Keywords: | Gouty nephropathy Yeast Adenine Kidney Rat |
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