| HSPgp96体外诱导腹腔巨噬细胞抗肿瘤细胞毒作用 |
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| 引用本文： | 施海燕,顾君一,张天一,林 琳,朱昌来. HSPgp96体外诱导腹腔巨噬细胞抗肿瘤细胞毒作用[J]. 中国肿瘤生物治疗杂志, 2005, 12(4): 272-274 |
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| 作者姓名： | 施海燕 顾君一 张天一 林 琳 朱昌来 |
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| 作者单位： | 1. 南通大学基础医学院病理生理学教研室,南通,226001
2. 南通大学神经再生重点实验室,南通,226001 |
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| 摘 要： | 目的:探讨HSPgp96对小鼠PEMφ体外抗肿瘤细胞毒作用的影响.方法:分离巯基乙酸盐诱导的小鼠PEMφ,随机分为培养液对照组、LPS诱导组、HSPgp96诱导组.应用硝酸还原酶法、MTT法、扫描电镜分别观测PEMφNO的生成、对肝癌细胞H22的细胞毒作用及形态学变化.结果:在HSPgp96的体外诱导下,小鼠PEMφNO的生成量显著增加,对肝癌细胞H22的细胞毒作用明显增强,其效应与LPS相当.结论:HSPgp96可在体外有效激活PEMφ的抗肿瘤细胞毒作用,NO是PEMφ杀瘤机制中的重要效应分子之一.
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| 关 键 词： | 热休克蛋白gp96 腹腔巨噬细胞 肿瘤 细胞毒作用 |
| 文章编号： | 1007-385X(2005)04-0272-03 |
| 收稿时间： | 2005-08-15 |
| 修稿时间： | 2005-10-09 |
The Cytotoxic Effect of Peritoneal Elicited Macrophages Induced by HSPgp96 on Anti-Tumor in vitro |
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| SHI Hai-yan,GU Jun-yi,ZHANG Tian-yi,LIN Lin and ZHU Chang-lai. The Cytotoxic Effect of Peritoneal Elicited Macrophages Induced by HSPgp96 on Anti-Tumor in vitro[J]. Chinses Journal of Cancer Biotherapy, 2005, 12(4): 272-274 |
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| Authors: | SHI Hai-yan GU Jun-yi ZHANG Tian-yi LIN Lin ZHU Chang-lai |
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| Affiliation: | 1. Department of Pathophysiology , College of Basic Medical Science, Nantong University, Nantong 226001, China ; 2. Key Laboratory of Neural Regeneration, Nantong University, Nantong 226001, China |
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| Abstract: | Objective: To evaluate the cytotoxic effect of PEM induced by HSPgp96 on anti-tumor in vitro. Methods:PEM separated from mice induced by thioglycolate were divided into three groups randomly: Culture medium in control; LPS-induced group; HSPgp96-induced group. The production of NO, the cytotoxic effect to H22 cells and the morphologic change of PEM were investigated separately by enzyme method, MTT assay and scanning electron microscope. Results:In vitro, HSPgp96 can increased NO production from PEM of mice and significantly enhance the cytotoxic effect of PEM to H22 cells as well as LPS. Conclusion:HSPgp96 can effectively induce the cytotoxic effect of PEM on anti-tumor in which NO is one of the capital effective molecules in vitro. |
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| Keywords: | heat shock protein gp96 peritoneal elicited macrophages tumor cytotoxicity |
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