| 人颌骨骨膜成骨细胞体外成骨的实验研究 |
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| 引用本文： | 藕小平,陈伟良,吴渺夷,张胜. 人颌骨骨膜成骨细胞体外成骨的实验研究[J]. 临床口腔医学杂志, 2009, 25(5) |
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| 作者姓名： | 藕小平 陈伟良 吴渺夷 张胜 |
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| 作者单位： | 东莞市人民医院口腔颌面外科,广东,东莞,523018;中山大学附属第二医院口腔颌面外科 |
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| 摘 要： | 目的:研究人下颌骨骨膜来源的种子细胞组织工程化成骨的可行性.方法:从人下颌骨骨膜内分离出成骨细胞,纯化后进行体外培养扩增,将其接种至异体部分脱钙松质骨支架内,复合物植入裸鼠皮下培养2个月,观察成骨细胞在支架内的粘附、生长和成骨情况.结果:从人下颌骨骨膜成功分离培养出成骨细胞,细胞在异体部分脱钙松质骨内粘附、生长,植入裸鼠皮下培养2个月后,能在支架内形成新生骨组织.结论:人下颌骨骨膜成骨细胞能在体外支架内组织工程化形成新生骨组织.
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| 关 键 词： | 骨组织工程 骨膜 成骨细胞 细胞培养 |
The experimental study on bone formation by human mandibular periosteum osteoblasts cutured in vitro |
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| OU Xiao-ping,CHEN Wei-liang,WU Miao-yi,ZHANG Sheng. The experimental study on bone formation by human mandibular periosteum osteoblasts cutured in vitro[J]. Journal of Clinical Stomatology, 2009, 25(5) |
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| Authors: | OU Xiao-ping CHEN Wei-liang WU Miao-yi ZHANG Sheng |
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| Affiliation: | OU Xiao-ping,CHEN Wei-liang,WU Miao-yi,ZHANG Sheng. Department of Oral , Maxillofacial Surgery,Dongguang Hos- pital,Guangdong Dongguan 523018,China |
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| Abstract: | Objective:To investigate the feasibility of tissue engineered bone by human mandibular periosteum osteoblasts cultured in vitro. Method:The osteoblasts isolated from human mandibular periosteum were cultured in vitro. Then they were seeded into alloplastic partially decalcified spong bone. The composite was implanted into subcutaneous pockets on the dorsum of athymic mice for 2 months after they were cultured in vitro for 4 days. In this course,the cells`growth,adhension and bone formation were observed. Re... |
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| Keywords: | bone tissue engineering periosteum osteoblast cell culture |
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