| ELISA法和MEIA法测定HBV血清标志物的比较研究 |
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| 引用本文： | 傅蕾,谭德明,彭仕芳,李铁刚,甘晓明.ELISA法和MEIA法测定HBV血清标志物的比较研究[J].中国现代医学杂志,2004,14(21):107-108. |
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| 作者姓名： | 傅蕾 谭德明 彭仕芳 李铁刚 甘晓明 |
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| 作者单位： | 中南大学湘雅医院,感染病科,湖南,长沙,410008 |
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| 摘 要： | 目的比较酶联免疫吸附法(ELISA)和微粒子酶免疫分析法(MEIA)对HBV血清标志物的检测性能.方法用ELISA法和MEIA法对HBV的血清标志物(HBsAg,HBeAg,Anti-HBc和Anti-HBe)分别进行检测.结果对1140例病人的血清进行乙肝标志物的检测,MEIA法阳性率高于ELISA法.与MEIA法相比较,ELISA法检测HBsAg的相对灵敏度达97.5%,Anti-HBc(94.9%)和HBeAg(73.4%)次之,Anti-HBe最低,为41.7%.对一些低滴度的HBeAg/Anti-HBe的HBV感染者,ELISA法可以引起假阴性.结论对于血清乙肝标志物的检测,MEIA法和ELISA法均有较高的特异性,但MEIA法敏感性优于ELISA法.
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| 关 键 词： | ELISA法 MEIA法 乙肝标志物 |
| 文章编号： | 1005-8982(2004)21-0107-02 |
Comparative study on ELISA and MEIA methods used in detecting serum HBV markers |
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| FU Lei,TAN De-ming,PENG Shi-fang,LI Tie-gang,GAN Xiao-ming.Comparative study on ELISA and MEIA methods used in detecting serum HBV markers[J].China Journal of Modern Medicine,2004,14(21):107-108. |
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| Authors: | FU Lei TAN De-ming PENG Shi-fang LI Tie-gang GAN Xiao-ming |
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| Abstract: | Objective:To compare the positive rates of ELISA and MEIA methods used in detecting serum HBV markers.Methods:ELISA and MEIA methods were used to detect HBV markers including HBsAg,HBeAg,Anti-HBc and Anti-HBe.Result:The positive rate of MEIA was higher than that of ELISA on detecting HBV marker of1140patients sera.Comparing with MEIA,ELISA had different relative sensitivities on detecting different markers,which were97.5%(HBsAg),94.9%(Anti-HBc),73.4%(HBeAg)and41.7%(Anti-HBe)respectively.Regarding HBV patients with low titers of HBeAg/Anti-HBe,ELISA could result in false negativity.Conclusions:MEIA is more sensitive than ELISA on the detection of serum HBV markers. |
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| Keywords: | ELISA MEIA HBV markers |
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