| MicroRNA-638在骨肉瘤中表达下调及对其细胞增殖的影响 |
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| 引用本文： | 陈斌,符勇,夏雪,郭伟明,金浩成,王晓旭.MicroRNA-638在骨肉瘤中表达下调及对其细胞增殖的影响[J].南华大学学报(医学版),2017(4):341-345, 363. |
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| 作者姓名： | 陈斌 符勇 夏雪 郭伟明 金浩成 王晓旭 |
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| 作者单位： | 南华大学附属第二医院骨科,湖南 衡阳 421001,南华大学附属第二医院骨科,湖南 衡阳 421001,南华大学附属第二医院骨科,湖南 衡阳 421001,南华大学附属第二医院骨科,湖南 衡阳 421001,南华大学附属第二医院骨科,湖南 衡阳 421001,南华大学附属第二医院骨科,湖南 衡阳 421001 |
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| 基金项目： | 湖南省教育厅资助项目(15C1215). |
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| 摘 要： | 目的 探讨miR-638在人骨肉瘤(OS)细胞增殖行为中的影响。方法通过实时定量聚合酶链反应(qRT-PCR)检测miRNA-638在人OS组织及癌旁组织、人OS细胞株(MG63、U2OS、HOS、SaOS2)和正常人成骨NHOst细胞株中的表达；用LipofectamineTM 2000转染miR-638模拟物或抑制物,细胞增殖检测试剂盒(CCK-8)检测转染后的吸光光度值,平板克隆形成实验检测菌落数量。结果miR-638在各组细胞中均有表达；OS细胞株中miR-638的表达明显下调(P＜0.05)；高表达miR-638能抑制OS MG63和U2OS细胞的增殖(P＜0.05)。 结论miR-638高表达能抑制OS细胞的增殖。
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| 关 键 词： | 骨肉瘤 MG63 U2OS MicroRNA-638 细胞增殖 |
| 收稿时间： | 2017/2/10 0:00:00 |
| 修稿时间： | 2017/6/18 0:00:00 |
Effect of MicroRNA-638 expression on the proliferation ofhuman osteosarcoma cells |
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| Institution: | Department of Orthopaedics,the Second Affiliated Hospital,University of South China,Hengyang,Hunan 421001,China,Department of Orthopaedics,the Second Affiliated Hospital,University of South China,Hengyang,Hunan 421001,China,Department of Orthopaedics,the Second Affiliated Hospital,University of South China,Hengyang,Hunan 421001,China,Department of Orthopaedics,the Second Affiliated Hospital,University of South China,Hengyang,Hunan 421001,China,Department of Orthopaedics,the Second Affiliated Hospital,University of South China,Hengyang,Hunan 421001,China and Department of Orthopaedics,the Second Affiliated Hospital,University of South China,Hengyang,Hunan 421001,China |
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| Abstract: | Objective To investigate the role of miR-638 in biological behavior of osteosarcoma cell.MethodsThe expression of miR-638 in osteosarcoma MG63 and U2OS cells was detected by RT-PCR.miRNA-638 mimics (inhibhors) were transfected into MG63 and U2OS by using LipofectamineTM2000 to upregulate(deregulate) the expression of miRNA-638.The absorbance value in 24 h,48 h,96 h and 72 h following the transfection was detected by CCK-8.And colony forming assay was used to detect colony counts over 30 cells after 2 weeks.ResultsMiR-638 expressed in each group.The expression of miR-638 was down regulated (P<0.001) in the 36 groups (56.25%) compared with the corresponding non cancerous tissues.Mir-638 expression can significantly reduce the MG63 and U2OS cells growth rate (P<0.05),on the contrary,the suppression of miR-638 expression significantly promoted the growth of cells of U2OS and MG63 (P<0.05).ConclusionsHigh expression of miR-638 significantly inhibits the proliferation of osteosarcoma cells.MiR-638 may become a new target for the early diagnosis and treatment of osteosarcoma. |
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| Keywords: | osteosarcoma MG63 U2OS MicroRNA-638 cell proliferation |
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