多层螺旋CT灌注参数与直肠癌微血管密度计数血管内皮生长因子表达的相关性研究

目的探讨多层螺旋CT灌注参数与直肠癌微血管密度(MVD)计数、血管内皮生长因子(VEGF)表达之间相关性。方法收集经病理活检证实的直肠癌患者59例。入选病例均使用GE64排螺旋CT机行直肠癌灌注扫描,获得血容量、血流量、平均通过时间(MTT)、表面通透性(PS)等灌注图像及其具体参数数值。并获得术后癌组织标本,采用CD34、VEGF单克隆抗体行免疫组织化学染色。分析多层螺旋CT灌注参数和MVD计数、VEGF表达情况的相关性。结果直肠癌组织VEGF表达、MVD计数与血流量、血容量呈正相关,与MTT呈负相关;与PS无相关性。结论多层螺旋CT灌注成像参数能够反应直肠癌肿瘤血管生成、VEGF表达及其血流动力学变化,为进一步研究直肠癌侵袭转移奠定了基础。

Correlation of MSCT perfusion parameters and MVD count of carcinoma of rectum and VEGF expression

Objective To investigate correlation MSCT perfusion parameters with MVD counts of carcinoma of rectum and VEGF expression.Methods Fifty nine cases of carcinoma of rectum patients were collected which is confirmed by biopsy.Cases were selected using the GE64 spiral CT perfusion scan of carcinoma of rectum.Acquiring the perfusion imaging and its specific parameter values such as the blood volume,blood flow,mean transit time,the permeability of surface.Carcinoma tissue samples were obtained after surgery,CD34,VEGF monoclonal antibody were used for immunohistochemical staining.Analyzing the correlation among MSCT perfusion parameters and MVD counts,VEGF expression.Results VEGF expression and MVD count in carcinoma tissues were positively correlated with BF and BV,negatively correlated with the MTT;There is no correlation with PS.Conclusion CT perfusion imaging parameters can reflect the carcinoma of rectum tumor angiogenesis,VEGF expression and hemodynamic changes,which laid the foundation for the further research of carcinoma of rectum invasion and metastasis.