5—HT6受体基因多态性与阿尔茨海默病的关联分析

目的探讨中国上海地区汉族人群中5-HT6受体基因多态性与阿尔茨海默病(AD)的相互关系.方法应用聚合酶链式反应(PCR)-限制性片段长度多态性(RFLP)方法,在106例AD患者,87例血管性痴呆(VD)患者和140例正常健康人中观察了5-HT6受体基因多态性的分布,并对5-HT6受体基因多态性与阿尔茨海默病之间的关系进行探讨.结果①阿尔茨海默病与5-HT6受体基因的多态性之间无显著意义的关联(P＞0.05);②在将受试人群进行ApoE基因分型后,ApoEε4型与非ApoEε4型人群中AD与5-HT6受体基因各基因型或等位基因均无关联(P＞0.05);③将AD患者进行ApoE基因分型后,非ApoEε4型AD与5-HT6的267C/T基因型正相关(OR=2.46,95%CI5.43-1.11,P＜0.05).结论中国上海地区汉族人群中5-HT6受体基因多态性与非ApoEε4型阿尔茨海默病相关联,表现为C/T型频率的升高.

The association study of 5-HT6 receptor gene polymorphism with Alzheimer's disease

Objectives: To detect the relation between 5-HT,receptor gene polymorphism and Alzheimer disease (AD) in Chinese. Method: The gene polymorphism of 5-HT6 receptor gene and ApoE were genotyped in 106 AD cases,87 VD cases and 140 controls with PCR methods and RFLP typing. Then the association between 5-HT5 receptor gene polymorphism and AD was analyzed. Results: 1 There was no association between AD and any alleles or genotypes of 5-HT6 receptor gene polymor-phism(P>0. 05); 2When the subjects were split into ApoEt4 positive and ApoEc4 negative groups, there was still no association between AD and any alleles or genotypes of 5-HT8 receptor gene polymorphism (P>0. 05) t 3When the AD cases were split into ApoEt4 positive and ApoEe4 negative groups, the 5-HT, 267C/T genotype showed strong association with non-ApoE,4 AD(OR = 2. 46, 95%CI:5. 43 - 1. 11,P<0. 05). Conclusion: The 5-HT8 receptor gene polymorphism may be associated with non-ApoEe4 AD in Han population of Shanghai area.