| 重组人乳头瘤病毒58型L1蛋白在Sf9细胞中的表达条件优化 |
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| 引用本文： | 于世荣,孙丽媛,刘君,丁军颖,李军,张夕燕,张振龙.重组人乳头瘤病毒58型L1蛋白在Sf9细胞中的表达条件优化[J].国际生物制品学杂志,2010,33(1):128-133. |
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| 作者姓名： | 于世荣 孙丽媛 刘君 丁军颖 李军 张夕燕 张振龙 |
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| 作者单位： | 北京生物制品研究所中试一室,100024; |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | Objective To select the optimum conditions for amplification and expression of the recombinant human papillomavirus 58 (HPV58) LI gene using baculovirus-insect cell expression system. Methods The Sf9 insect cells at logarithmic phase was used to amplify virus at different multiplicities of infection (MOI). Titers of the virus were determined by plaque assay. The expression of recombinant protein was identified by indirect immunofluorescence assay, and the expression of recombinant protein at different MOI and time was determined by Western blot. Results Recombinant baculovirus containing HPV58 LI gene were amplified at MOI of 0.5 for 48 h when the Sf9 insect cells were at logarithmic phase, and the highest titers of the virus reached 5×108 pfu/ml. The optimum conditions for expressing the recombinant protein were that the recombinant baculovirus was inoculated at MOI of 5.0 and the protein was expressed within 72 h. Conclusion The optimum conditions for amplifying the virus and expressing the recombinant protein are determined.
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| 关 键 词： | 人乳头瘤病毒 基因表达 重组蛋白质类 |
Optimization of conditions for expressing recombinant HPV58 L1 protein in Sf9 insect cells |
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| YU Shi-rong,SUN Li-yuan,LIU Jun,DING Jun-ying,LI Jun,ZHANG Xi-yan,ZHANG Zhen-long.Optimization of conditions for expressing recombinant HPV58 L1 protein in Sf9 insect cells[J].International Journal of Biologicals,2010,33(1):128-133. |
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| Authors: | YU Shi-rong SUN Li-yuan LIU Jun DING Jun-ying LI Jun ZHANG Xi-yan ZHANG Zhen-long |
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| Abstract: | |
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| Keywords: | Human papillomavirusGene expressionRecombinant proteins |
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