三种水溶性壳聚糖的制备工艺研究

目的 壳聚糖具有优良的生物相容性和生物可降解性,但其亲水性较差难以满足组织工程的需要.为满足组织工程支架材料的需求,对壳聚糖进行羧甲基化改性,以期改善其溶解性能.方法 通过3种不同的方法来摸索3种不同取代位置的羧甲基壳聚糖(CMC)的制备工艺,分别考察了原料比、反应时间、反应温度等影响因素对产物取代度(DS)的影响.结果 摸索得到3种羧甲基壳聚糖的最佳制备工艺条件,以这种工艺条件制备产物水溶性良好.结论 通过羧甲基化反应可以大大改善壳聚糖的水溶解性能,为以后更深一步的研究提供了科研基础和实验依据.

Preparation of three types soluble chitosan

Objective Although chitosan has excellent biocompatibility and biodegradability, it is only soluble in acid solution that is not feasible for application in tissue engineering. The current study is aimed to prepare the carboxymethyl chitosan (CMC) for the purpose of improving the solubility of chitosan to meet the requirement of development of tissue engineering scaffolds. Methods The preparation methods and process conditions, such as the proportion of reactant, reaction temperature, reaction time and so on for three types of carboxymethyl chitosan were studied. Results The results indicated that optimal process conditions of three types of water soluble chitosan were obtained. Conclusion Water solubility of chitosan is greatly improved through carboxymethyl modification, which provides possibilities and foundation for further research.