新生小鼠睾丸组织移植到裸鼠体内不同时期移植物的组织学观察

目的 通过测量不同发育时期移植物的重量及观察其中生精小管结构和生精细胞组成情况,对去势雄性小鼠中移植的新生小鼠睾丸组织生长和发育进行系统观察.方法 将出生1～2d昆明小鼠睾丸移植到7～12周去势雄性免疫缺陷小鼠背部;在移植后不同时间段 (分为3d、1～11周和3～6月16个组)取出移植物,计算移植物的回收率,测定移植物重量,观察移植物中生精小管结构及生精细胞的组成.结果 从移植的450个新生小鼠睾丸组织,回收到405个移植物,总回收率为90.0%.重量比移植前增加约40倍.移植物中生精小管的发育及各阶段生精细胞出现时间与在正常小鼠中所见基本相同.移植时间超过8周后,生精上皮的退化现象显著增加.结论 将新生小鼠睾丸组织异位移植到受体背部后的发育进程与在体情况相同,第1次生精波结束后的时间应为获取精子细胞和精子的最佳时间,大约是移植后5～7周.

THE HISTOLOGICAL OBSERVATION OF THE STEPWISE DEVELOPMENT OF NEONATAL MOUSE TESTIS IN IMMUNODEFICIENT MICE

Objective To investigate the development of neonatal mouse testis in castrated immunodeficient mice by monitoring the graft survival and weight and observing the structure of seminiferous epithelium and the composition of spermatogenic cells in grafts. Methods Neonatal Kun-ming mouse testis were grafted under the skin of castrated nude mice(7-12week-old).Grafts were then taken out at different time intervals(namely 16 time points: 3 days,1-11 weeks respectively and 3-6 months respectively).The survival rate of grafts was calculated and the wet weight was measured.Histological analysis was performed for the observation of the structure of seminiferous epithelium and the composition of spermatogenic cells in grafts. Results Four hundrcd and five grafts recovered out of 450 testis grafted, resulting in a recovery rate of 90.0%.The graft weight increased more than 40 times.The developmental pattern of seminiferous tubules and the appearance time of various spermatogenic cells in grafts were similar as seen in intact mice.Eight weeks after the grafrting,an increasing degradation of seminiferous epithelium was observed.Conclusion When neonatal mouse testis were grafted into nude mice,the developmental course was similar as that in normal donors.The optimal retrieval time of round spermatids and sperms was around the end of the first spermatogenesis wave,about 5-7weeks after the grafting procedure.