昆明彝族人群HLA-DRB1、DQB1基因多态性

目的　调查昆明彝族 HL A- DRB1、DQB1基因的多态性。方法　应用聚合酶链反应 -序列特异性引物基因分型技术 ,对昆明地区 70名彝族健康儿童进行了 HL A- DRB1、DQB1位点的基因分型。结果在 HL A- DRB1位点共检出了 12种等位基因 ,其中等位基因频率大于 10 %的依次为 HL A- DRB1* 12 (33.5 7% )、DRB1* 0 90 1(11.4 3% )、DRB1* 0 4 (11.4 3% ) ,等位基因频率大于 5 %而小于 10 %的依次为 DRB1* 0 1(8.5 7% )、DRB1* 11(7.86 % )、DRB1* 14 (7.14 % )、DRB1* 15 (7.14 % )、DRB1* 0 8(5 % ) ,等位基因频率小于 5 %依次为 HL A- DRB1* 0 3(2 .86 % )、DRB1* 13(2 .14 % )、DRB1* 0 7(1.4 3% )、DRB1* 16 (1.4 3% )。在 HL A- DQB1位点共检出了 7种等位基因 ,其中等位基因频率大于 10 %的依次为 HL A- DQB1*0 30 1(45 % )、DQB1* 0 5 (2 2 .14 % )、DQB1* 0 30 3(12 .14 % ) ,等位基因频率大于 5 %而小于 10 %的依次为DQB1* 0 4 (6 .4 3% )、DQB1* 0 6 (6 .4 3% ) ,等位基因频率小于 5 %的依次为 DQB1* 0 2 0 1(4.2 9% )、DQB1* 0 30 2 (3.5 7% )。结论　昆明彝族 HL A基因多态性分布不同于北方汉族人群 ,也不同于南方汉族人群 ,有其独特性。

Analysis of HLA-DRB1,DQB1 allele polymorphism in the Kunming Yi nationality population

Objective To investigate the HLA-DRB1,DQB1 allele polymorphism in Kunming Yi nationality population. Methods HLA-DRB1, DQB1 DNA types in 70 healthy children of Yi nationality in Kunming were analyzed by polymerase chain reaction with sequence specific primer (PCR-SSP). Results Twelve alleles at HLA-DRB1 locus were observed in the 70 children: the alleles with gene frequencies higher than 10% were HLA-DRB1*12(33.57%),DRB1*0901(11.43%),DRB1*04(11.43%); the alleles with gene frequencies between 10% and 5% were HLA-DRB1*01(8.57%),DRB1*11(7.86%),DRB1*14(7.14%),DRB1*15(7.14%) ,DRB1*08(5%); the alleles with gene frequencies lower than 5% were HLA-DRB1*03(2.86%),DRB1*13(2.14%),DRB1*07(1.43%),DRB1*16(1.43%). Seven alleles at HLA-DQB1 locus were observed in the 70 children: the alleles with gene frequencies higher than 10% were HLA-DQB1*0301(45%),DQB1*05(22.14%),DQB1*0303(12.14%); the alleles with gene frequencies between 10% and 5% were HLA-DQB1*04(6.43%),DQB1*06(6.43%); the alleles with gene frequencies lower than 5% were HLA-DQB1*0201(4.29%) and DQB1*0302(3.57%). Conclusion The distribution of HLA-DRB1,DQB1 allele polymorphism in the Kunming Yi nationality population is distinctive. It is neither like that in the South Han population nor like that in the North Han population.