| 鲎试剂方法检测注射用复合辅酶中细菌内毒素 |
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| 引用本文： | 王宗春,姜俊勇,宋金春. 鲎试剂方法检测注射用复合辅酶中细菌内毒素[J]. 中国医药导刊, 2011, 13(8): 1452-1453 |
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| 作者姓名： | 王宗春 姜俊勇 宋金春 |
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| 作者单位： | 武汉大学人民医院药学部,武汉,430060 |
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| 摘 要： | 目的:建立注射用复合辅酶细菌内毒素检查法。方法:按照中国药典2010年版(二部)附录ⅪE细菌内毒素检查法进行干扰试验。结果:用灵敏度λ为0.5EU/ml的鲎试剂进行实验,当注射用复合辅酶中辅酶Ⅰ的浓度为0.8μg/ml时,供试品不干扰鲎试剂与细菌内毒素的凝集反应。结论:使用鲎试剂方法检查注射用复合辅酶中细菌内毒素是可行的。
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| 关 键 词： | 注射用复合辅酶 细菌内毒素 鲎试剂 |
Detection of Bacterial Endotoxins in Coenzyme Complex for Injection by Tachypleus Amebocyte Lysate Test |
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| Wang Zong-chun,Jiang Jun-yong,Song Jin-chun. Detection of Bacterial Endotoxins in Coenzyme Complex for Injection by Tachypleus Amebocyte Lysate Test[J]. Chinese Journal of Medicinal Guide, 2011, 13(8): 1452-1453 |
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| Authors: | Wang Zong-chun Jiang Jun-yong Song Jin-chun |
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| Affiliation: | (Dept.of Pharmacy,People’s Hospital of Wuhan University,Wuhan 430060,China) |
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| Abstract: | Objective:To establish a method for the detection of bacterial endotoxins in coenzyme complex for injection. Methods:The interference test was carried out in accordance with appendixⅪE.volume two,chinese pharmacopoeia 2010 edition. Results:By using tachypleus amebocyte lysates whose sensitivity was 0.5 EU/ml,the sample did not interfere the congregating reactivity of tachypleus amebocyte lysate with bacterial endotoxins when the concemtration of coenzyme I was below 0.8μg/ml.Conclusion:The method of tachypleus amebocyte lysate test is feasible for detecting the bacterial endotoxins in coenzyme complex for injection. |
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| Keywords: | Coenzyme Complex for Injection Bacterial Endotoxins Tachypleus Amebocyte Lysate |
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