褪黑素对慢性间歇性缺氧心肺保护作用

目的:探讨褪黑素(MT)对常压下慢性间歇缺氧损伤SD大鼠心肺的保护作用。方法:将24只雄性,5周SD大鼠随机分成三组并模拟人类睡眠时间:对照组在常压常氧下饲养加灌服生理盐水,实验组分为实验1组常压低氧箱内饲养加灌服褪黑素、实验2组在常压低氧箱内饲养同时加灌服生理盐水,8周后检测体重(BW)、右心室/左心室加室间隔比值RV/(LV+S),右心室/体质量(RV/BW)比值以及心肌和肺组织谷胱苷肽过氧化物酶(GSH-PX)活力、超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)的变化。结果:实验2组与实验1组比较大BW明显降低(P<0.05),而RV/(LV+S)及RV/BW明显增加(P<0.05),心肌及肺组织GSH-PX活力、SOD活性、T-AOC均明显降低(P<0.05);实验1组与对照组比较BW无明显差异(P>0.05),而RV/(LV+S)及RV/BW无明显差异(P>0.05),心肌及肺组织GSH-PX活力、SOD活性、T-AOC均无明显差异(P>0.05);实验2组与对照组比较BW明显降低(P<0.05),而RV/(LV+S)及RV/BW却增加(P<0.05),心肌及肺组织GSHPX活力、SOD活性、T-AOC均明显差异(P<0.05)。结论:褪黑素可防止慢性间歇缺氧对心肺的损伤作用。

Protective Effect of Melatonin on Chronic iIntermittent Hypoxia Cardiopulmonary Tissue

Objective:To investigate that the melatonin(MT)could protect SD rats’ cardiopulmonary tissue from the chronic intermittent hypoxia damage in the Atmospheric pressure.Methods:The 24 male,5 weeks SD rats were randomly divided into three groups.And simulated human sleep time:8 rats were sent into the control group.And the control group rats wre fed with normal saline solution(NS)in the normal oxygen pressure;16 experments rats were divided into two groups avergely:experiment group 1 and experiment group 2.The experiment group 1 rats wre fed with melatonin(MT)in the hyposia box;the experiment group 2 rats wre fed with normal saline solution(NS)in the hypoxia box;After 8 weeks each rat weight was recorded,the ratio of the right ventricle/left ventricle plus septum[RV/(LV+S)],the ratio of the right ventricle/body weight(RV/ BW).And detected the Glutathione peroxidase(GSH-PX)activity,superoxide dismutase(SOD)activity,total antioxidant capacity(T-AOC)of every rat’s myocardial and lung tissue.Result:The weights of experiment group 1 rats’ were significantly increasing than experiment group 2’ s(P<0.05),the RV/(LV+S)and RV/BW of experiment group 1 rats’ were significantly lower than experiment group 2’s(P<0.05),the glutathione peroxidase(GSH-PX)activity,superoxide dismutase(SOD)activity and total antioxidant capacity(T-AOC)of experiment group 1 rats’ heart and lung tissue were significantly increasing than experiment group 2’s(P<0.05).The above indicators had no obvious difference between experiment group 1’s and the control group’s(P>0.05),but the weights of the experiment group 2 were significantly lower the control group’s(P<0.05). the RV/(LV+S)and RV/BW of experiment group 2 were significantly increasing than the control group’s(P>0.05).The another indicators had significantly difference between experiment group 2’s and the control group’s.Conclusion:The chronic intermittent hypoxia could damage SD rats’ heart and lung.Melatonin(MT)could prevent cardiopulmonary tissue from the chronic intermittent hypoxia damage.