基质金属蛋白酶-9启基因启动子区单核苷酸多态性与早产易感性的关系

目的:研究基质金属蛋白酶-9(matrix metalloproteinases-9,MMP-9)与中国北方妇女早产遗传易感性的关系。方法:采用聚合酶链反应-限制性片段长度多态性分析(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法,检测100例早产患者和100例对照组妇女的MMP-9基因启动子区-1562C/T单核苷酸多态性位点的基因型。结果:早产患者组和对照组的MMP-9基因型分布均符合Hardy-Weinberg平衡(P>0.05)。早产患者MMP-9中C和T等位基因频率在病例组和对照组分别为89.00%,11.00%及92 50%,7.50%,两组差异无统计学意义(χ~2=1.46,P=0.23);病例组C/C,C/T和T/T基因型频率分别为79.00%,20.00%和1.00%,对照组分别为85.00%、15.00%和0.00%,两者差异亦无统计学意义(χ~2=1.220,P=0.27)。与C/C基因型相比,携带T等位基因未能明显增加早产的发病风险,经年龄校正的OR值为1.52(95%CI=0.78～3.10)。结论:MMP-9启动子区-1562C/T多态性可能与早产的发病风险无关。

Association of the SNP in the Matrix Metalloproteinase-9 Promoter Polymorphism with Susceptibility of Preterm Birth

Objective:The aim of this study was to investigate the association of the MMP-9 SNP with susceptibility of preterm birth in North Chinese.Methods:Genotypes of the MMP-9 promoter SNP were performed in 100 preterm birth patients and 100 healthy controls by polymerase chain reaction-restriction fragment length polymorphism.Results:The MMP-9 genotype distribution in both preterm birth patients and healthy controls did not significantly deviated from that expected by Hardy-Weinberg equilibrium(P>0.05).The frequency of the C and T allele among preterm birth patients and healthy controls were 89.00%,11.00%and 92.50%,7.50%,respectively.No significant difference in MMP-9 allele distribution was shown between cases and controls(χ~2=1.46,P=0.23).The genotype frequencies of C/C,C/T,T/T in the case were 79.00%,20.00%and 1.00%.While the genotype frequencies of C/C,C/T,T/T in the control were 85.00%,15.00%and 0.00%.No significant difference of genotype distribution was observed between preterm birth patients and healthy women(χ~2=1.22,P=0.27).Compare with the C homozygotes,carriers of the T allele was not significantly modified the risk of developing preterm birth,the adjusted odds ratio was 1.52(95%CI= 0.78～3.10).Conclusion:MMP-9 promoter SNP was not association with risk of preterm birth.