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| Free radicals and antioxidant systems in reflux esophagitis and Barrett＇s esophagus | |
| 引用本文： | Jiménez P,Piazuelo E,Sánchez MT,Ortego J,Soteras F,Lanas A. Free radicals and antioxidant systems in reflux esophagitis and Barrett＇s esophagus[J]. World journal of gastroenterology : WJG, 2005, 11(18): 2697-2703 |
| 作者姓名： | Jiménez P Piazuelo E Sánchez MT Ortego J Soteras F Lanas A |
| 作者单位： | Instituto Aragons de Ciencias de la Salud and Service of Gastroenterology Hospital Clinico Universitario "Lozano Blesa" Avenida San Juan Bosco 15 50009 Zaragoza Spain,Instituto Aragon茅s de Ciencias de la Salud and Service of Gastroenterology Hospital Clinico Universitario "Lozano Blesa" Avenida San Juan Bosco 15 50009 Zaragoza Spain,Instituto Aragon茅s de Ciencias de la Salud and Service of Gastroenterology Hospital Clinico Universitario "Lozano Blesa" Avenida San Juan Bosco 15 50009 Zaragoza Spain,Service of Pathology Hospital Cnico Universitario "Lozano Blesa" Avenida San Juan Bosco 15 50009 Zaragoza Spain,University of Zaragoza Domingo Miral s/n.50009 Zaragoza Spain |
| 基金项目： | Supported by the grant FIS 99/0569 from the Fondo de Investigaciones Sanitarias and Instituto de Salud Carlos Ⅲ (C03/02) |
| 摘 要： | AIM: Experimental studies suggest that free radicals are involved in acid and pepsin-induced damage of esophageal mucosa. The profile and balance between free radicals and antioxidant systems in human esophagitis are unknown. METHODS: Superoxide anion and its powerful oxidant reaction with nitric oxide (peroxynitrite) generation were determined in esophageal mucosal biopsies from 101 patients with different gastro-esophageal reflux diseases and 28 controls. Activity of both superoxide dismutase (SOD) and catalase, and reduced glutathione (GSH) levels, were also assessed. Expression of Cu,ZnSOD, MnSOD and tyrosine-nitrated MnSOD were analyzed by Western blot and/or immunohistochemistry. RESULTS: The highest levels of superoxide anion generation were found in patients with severe lesions of esophagitis. Peroxynitrite generation was intense in Barrett's biopsies, weaker in esophagitis and absent/weak in normal mucosa. Expression of Cu,ZnSOD and MnSOD isoforms were present in normal mucosa and increased according to the severity of the lesion, reaching the highest level in Barrett's esophagus. However, SOD mucosal activity significantly decreased in patients with esophagitis and Barrett's esophagus, which was, at least in part, due to nitration of its tyrosine residues. Catalase activity and GSH levels were significantly increased in mucosal specimens from patients with esophagitis and/or Barrett's esophagus. CONCLUSION: A decrease in SOD antioxidant activity leading to increased mucosal levels of superoxide anion and peroxynitrite radicals may contribute to the development of esophageal damage and Barrett's esophagus in patients with gastroesophageal reflux. Administration of SOD may be a therapeutic target in the treatment of patients with esophagitis and Barrett's esophagus. |
| 关 键 词： | 自由因子 抗氧化剂 逆流性食道炎 巴雷特氏食道疾病 病理机制 |
Free radicals and antioxidant systems in reflux esophagitis and Barrett's esophagus |
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| Jiménez Pilar,Piazuelo Elena,Sánchez M Teresa,Ortego Javier,Soteras Fernando,Lanas Angel. Free radicals and antioxidant systems in reflux esophagitis and Barrett's esophagus[J]. World journal of gastroenterology : WJG, 2005, 11(18): 2697-2703 | |
| Authors: | Jiménez Pilar Piazuelo Elena Sánchez M Teresa Ortego Javier Soteras Fernando Lanas Angel |
| Affiliation: | 1. Instituto Aragonés de Ciencias de la Salud and Service of Gastroenterology, Hospital Clínico Universitario "Lozano Blesa", Avenida San Juan Bosco, 15 50009 Zaragoza, Spain 2. Service of Pathology, Hospital Clínico Universitario "Lozano Blesa", Avenida San Juan Bosco, 15 50009 Zaragoza, Spain 3. University of Zaragoza, Domingo Miral, s/n. 50009 Zaragoza, Spain |
| Abstract: | AIM: Experimental studies suggest that free radicals are involved in acid and pepsin-induced damage of esophageal mucosa. The profile and balance between free radicals and antioxidant systems in human esophagitis are unknown. METHODS: Superoxide anion and its powerful oxidant reaction with nitric oxide (peroxynitrite) generation were determined in esophageal mucosal biopsies from 101 patients with different gastro-esophageal reflux diseases and 28 controls. Activity of both superoxide dismutase (SOD) and catalase, and reduced glutathione (GSH) levels, were also assessed. Expression of Cu,ZnSOD, MnSOD and tyrosine-nitrated MnSOD were analyzed by Western blot and/or immunohistochemistry. RESULTS: The highest levels of superoxide anion generation were found in patients with severe lesions of esophagitis. Peroxynitrite generation was intense in Barrett's biopsies, weaker in esophagitis and absent/weak in normal mucosa. Expression of Cu,ZnSOD and MnSOD isoforms were present in normal mucosa and increased according to the severity of the lesion, reaching the highest level in Barrett's esophagus. However, SOD mucosal activity significantly decreased in patients with esophagitis and Barrett's esophagus, which was, at least in part, due to nitration of its tyrosine residues. Catalase activity and GSH levels were significantly increased in mucosal specimens from patients with esophagitis and/or Barrett's esophagus. CONCLUSION: A decrease in SOD antioxidant activity leading to increased mucosal levels of superoxide anion and peroxynitrite radicals may contribute to the development of esophageal damage and Barrett's esophagus in patients with gastroesophageal reflux. Administration of SOD may be a therapeutic target in the treatment of patients with esophagitis and Barrett's esophagus. |
| Keywords: | Superoxide anion Peroxynitrite Antioxidant enzymes Esophagitis Barrett's esophagus |
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