GSTM1和GSTT1基因多态性与女性肺癌易感性的关系

目的：探讨谷胱苷肽硫转移酶M1（glutathione S-transferaseM1,GSTM1)和T1（glutathione S-transferaseT1,GSTT1)基因多态性与女性肺癌遗传易感性的关系。方法：采用病例－对照研究方法和多重PCR技术检测女性肺癌病例组42人和健康对照组55人的GSTM1和GSTT1基因缺陷型的频率，并评价GSTM1和GSTT1基因型以及他们之间的交互作用与肺癌遗传易感性的关系。结果：在本次研究的人群中，病例组GSTM1和GSTT1基因缺陷型的频率分别为66．7％和45．2％，对照组为54．5％和38．2％，GSTM1基因缺陷型和GSTT1基因缺陷型的频率在病例组和对照组之间均无显性差异（P＞0．05）。在不吸烟的女性人群中，GSTM1基因缺陷型携带患肺癌的危险性是GSTM1基因功能型携带的2．557倍（P＝0．046）；GSTT1基因缺陷型则有女性肺癌的发生无显关联（P＝0．557）。此外，GSTT1基因型与GSTM1基因型之间亦无明显的交互作用（P＞0．05）。结论：GSTM1基因缺陷型可能是非吸烟女性患肺癌的重要危险因素。GSTT1基因缺失则可能与肺癌的发生无关，在女性肺癌的发生过程中GSTM1和GSTT1可能不存在交互作用。

Study on Genetic Polymorphisms of GSTM1 and GSTT1 Related with Inherent Susceptibility to Lung Cancer in Women

Objective To examine the relationship of glutathione S transferase M1(GSTM1) and T1(GSTT1) with the susceptibility to lung cancer in women.Methods The case control study was conducted among 42 lung cancer and 55 healthy controls.The genetic polymorphisms of GSTM1 and GSTT1 were detected with the method of multiplex polymerase chain reaction.Stratification analysis was used to assess the interaction between GSTM1 and GSTT1 null genotypes.Results The frequences of GSTM1 and GSTT1 null genotypes were 66 7% and 45 2% respectively in lung cancer,while 54 5% and 38 2% in healthy controls.There was no obvious difference between lung cancer and healthy controls( P >0 05).But in nonsmoking women subjects the frequence of GSTM1 null genotype was significantly different between lung cancer and healthy controls( P =0 046),and the null genotype was associated with an increased risk of lung cancer in nonsmoking women.But GSTT1 null genotype was not overrepresented in non smoking women with lung cancer( P =0 557) compared with controls.The results from interaction analysis showed GSTM1 null genotypes combined with GSTT1 null genotypes did not have interaction on the risk of lung cancer.Conclusion GSTM1 deletion may be an important host risk for lung cancer in nonsmoking women,whereas GSTT1 deletion is not associated with the risk to lung cancer in women.Furthermore,there was no significant interaction between GSTM1 and GSTT1 null genotypes which could affect the susceptibility of women to lung cancer.