聚乳酸与重组人骨形成蛋白-2复合植入块修复兔下颌骨缺损的早期观察

采用冷冻干燥法，将聚乳酸（ＰＬＡ）制成多孔成形块状，并将ＰＬＡ与重组人骨形成蛋白－２（ｒｈＢＭＰ－２）有效的复合，植入兔下颌骨缺损动物模型，在术后２，４周通过Ｘ线片、组织学观察缺损部位的骨生成情况。结果表明：ＰＬＡ－ｒｈＢＭＰ－２植入组术后２周就有部分新骨形成，术后４周骨生成明显；而单独植入ＰＬＡ组术后４周仅有少量新骨生成。钙含量测定显示ＰＬＡ－ｒｈＢＭＰ－２组高于ＰＬＡ组。结果提示：ＰＬＡ为ＢＭＰ的有效传递系统，ＰＬＡ－ｒｈＢＭＰ－２复合植入块是一种有应用潜能的人工骨替代物。

Early Observation of Healing of Rabbit Mandibular Defects with Porous Blocks Consisting of Polylactic Acid and Recombinant Human Bone Morphogenetic Protein 2

The effects of bone morphogenetic protein(BMP) which can induce bone formation have been surely proved, Porous polylactic acid blocks were prepared by a freeze drying method. During preparation of the blocks, recombinant human bone morphogenetic protein 2(rhBMP 2)was mixed with the polylactic acid(4 mg per block). PLA rhBMP 2 was implanted in the mandibular defects of rabbit and retrieved after 2 or 4 weeks. The results showed that: with the PLA rhBMP 2 blocks, new bone formation was observed in 2 weeks after implantation, and plain PLA blocks produced a little of new bone formation in the edges of defect even in 4 weeks after implantation. Calcium content of the retrieved PLA rhBMP 2 block was statistically higher than that of plain PLA blocks. These results suggest that PLA may be effective delivery system for BMP and the PLA/rhBMP 2 block may potentially have usefulness as a bone graft substitute.