一起甲型副伤寒沙门菌污染水源所致腹泻暴发疫情的病原学调查

目的对一起腹泻暴发疫情进行病原学调查,以便明确病因、进行有效的预防控制。方法采用荧光PCR快速检测、常规病原学分离培养、噬菌体裂解试验、ATB微生物自动鉴定系统等方法对疑似患者、环境水源水和饮用水采样,进行病原学的检测和鉴定。结果经荧光PCR检测与病原分离培养鉴定,来自患者粪便、血液、水源水和饮用水共12份疑似标本中,10份为甲型副伤寒沙门菌阳性,检出率为83.33%,其中4份来自粪便标本,4份来自血,1份来自水源水,1份来自饮用水。10株不同来源甲型副伤寒沙门菌的血清学分型、生化反应、药物敏感性、噬菌体裂解试验均相同。结论这是一起由甲型副伤寒沙门菌水源污染所致的腹泻暴发疫情。该菌的检出为本地区腹泻病原谱增加了一种新的病原菌。

Etiological survey on a diarrhea outbreak epidemic caused by water source contaminated by Salmonella paratyphi A

Objective An etiological survey was conducted on a diarrhea outbreak epidemic to make clear of the cause and implement effective prevention and control.Methods Fluorescence PCR rapid detection,normal etiological separation and cultivation,phagelysis experiments,ATB automated identification system were used to make etiological detection and identification on probable and suspected cases,environmental water source and drinking water source samples.Results Through fluorescence PCR detection and etiological separation,cultivation and identification,in suspected samples from patient feces,blood,water source and drinking water,10 samples were Salmonella paratyphi A positive with 83.33% detection rate,in which 4 samples from feces,4 samples from blood,one sample from water source and one sample from drinking water.The ten isolates from different sources showed the same serological subtype,biochemical reaction,drug sensitivity and phage lysis experiments of Salmonella paratyphi.Conclusion This was a diarrhea outbreak epidemic caused by water source contaminated by Salmonella paratyphi A.The detection of this bacterium added a new type of etiological bacteria in diarrhea etiological mapping in this district.