河南省有偿供血人群HIV/HCV混合感染者HIV-1细胞嗜性的研究

目的 探讨河南省有偿供血人群HIV/HCV混合感染者体内HIV-1细胞嗜性的分布情况.方法 选取河南省经有偿供血途径感染HIV-1的161例感染者,采用酶联免疫法检测HCV抗体,并通过HCV核酸检测明确HIV/HCV混合感染.nested-PCR扩增env C2V3区,测序并分析HIV -1流行亚型,使用在线预测工具W...

HIV-1 cell tropism of the population coinfected with HIV and HCV through paid blood donation in Henan province

Objective To investigate HIV-1 cell tropism of the population coinfected with HIV and HCV through paid blood donation in Henan province.Methods Totally 161 HIV-1 sero-positive blood samples were collected from individuals who were coinfected with HIV and HCV through paid blood donation in Henan province.HCV antibody was determined using enzyme-linked immunosorbent assay,and HIV/HCV coinfection was confirmed by the detection of HCV RNA.HIV-1 env C2V3 gene was amplified using nested-PCR and the subtype characterization was analyzed after sequencing.Co-receptor usage was predicted using biosoftware WebPSSM,and distribution of tetramer compositions on the tip of V3 loop was analyzed.Results Among the total 161 individuals infected with HIV-1 through paid blood donation,HCV sero-prevalence was 87.58%（141/161）,and the positive rate of HCV RNA was 56.52%（91/161）.The most predominant HIV-1 subtypes in both HIV monoinfection group and HIV/HCV coinfection group were B（B＇）（96% and 98.5%,respectively）,and the main tip motifs of V3 loop were GPGR（33.4% and 33.8%）,GPGQ（22.0% and 19.1%） and GPGK（14.0% and 19.1%）.Co-recepter CCR5 was mainly used in both HIV monoinfection group and HIV/HCV coinfection group,and the HIV-1 cell tropism between the two groups was not significantly different（P=0.971）.Conclusions The cell tropism of HIV-1 has no correlation with HCV infection.The decetion of HCV RNA helps to clarify the current infection situation in HIV/HCV coinfected population.