|
|||||
|
|
Development and characterization of 29 polymorphic microsatellite loci for Esomus metallicus |
|
| Authors: | Noriyuki Koizumi Shinsuke Morioka Thomas W. Quinn Atsushi Mori Bounsong Vongvichith Kazuya Nishida Keiji Watabe Takeshi Takemura |
| Affiliation: | 1. Institute for Rural Engineering, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, 305-8609, Japan 2. Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, 305-8686, Japan 3. Department of Biological Sciences, University of Denver, Denver, CO, 80208, USA 4. Living Aquatic Resources Research Center, Vientiane, 9108, Lao PDR |
| Abstract: | Twenty-nine microsatellite loci were isolated and characterized from the genome of Esomus metallicus. Flanking polymerase chain reaction primers were designed and used to amplify these loci in 32 individuals. All loci were polymorphic; with allele numbers ranged from 2 to 27, observed heterozygosity from 0.031 to 0.969 and expected heterozygosity from 0.031 to 0.957. All conformed to Hardy?CWeinberg equilibrium and no evidence of null alleles was observed. Pairwise comparisons between alleles did not detect any cases of linkage disequilibrium. The high level of polymorphisms observed in these microsatellite loci should enhance future investigation of the genetic differentiation and structure of populations of E. metallicus. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |