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Isolation and characterization of 14 microsatellite markers for Rhizophora mucronata (Rhizophoraceae) and their potential use in range-wide population studies |
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| Authors: | Yoshimi Shinmura Alison K. S. Wee Koji Takayama Sankararamasubramanian Halasya Meenakshisundaram Takeshi Asakawa Onrizal Bayu Adjie Erwin Riyanto Ardli Sarawood Sungkaew Norhaslinda Binti Malekal Nguyen Xuan Tung Severino G. Salmo III Orlex Baylen Yllano M. Nazre Saleh Khin Khin Soe Emiko Oguri Noriaki Murakami Yasuyuki Watano Shigeyuki Baba Edward L. Webb Tadashi Kajita |
| Affiliation: | 1. Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan 2. Department of Biological Sciences, National University of Singapore, Singapore, Singapore 3. Department of Systematic and Evolutionary Botany, University of Vienna, Vienna, Austria 4. Biotechnology Programme, M.S. Swaminathan Research Foundation, Chennai, India 5. Forestry Sciences Department, Universitas Sumatera Utara, Medan, Indonesia 6. Bali Botanical Garden, Indonesian Institute of Sciences, Bali, Indonesia 7. Faculty of Biology, Jenderal Soedirman University, Purwokerto, Indonesia 8. Forest Biology Department, Faculty of Forestry, Kasetsart University, Bangkok, Thailand 9. Institute for Tropical Biology and Conservation, Universiti Malaysia Kota Kinabalu, Kota Kinabalu, Malaysia 10. Mangrove Ecosystem Research Centre, Hanoi National University of Education, Hanoi, Vietnam 11. Department of Environmental Science, School of Science and Engineering, Ateneo de Manila University, Quezon City, Philippines 12. Biology Department, College of Sciences and Technology, Adventist University of the Philippines, Cavite, Philippines 13. Department of Forest Production, Faculty of Forestry, Universiti Putra Malaysia, Serdang, Selangor, Malaysia 14. Department of Botany, University of Yangon, Yangon, Myanmar 15. Makino Herbarium, Department of Biological Sciences, Tokyo Metropolitan University, Tokyo, Japan 16. Tropical Biosphere Research Center, University of the Ryukyus, Iriomote, Okinawa, Japan |
| Abstract: | A set of 14 new microsatellite markers was developed for mangrove species Rhizophora mucronata (Rhizophoraceae) by using pyrosequencing. Fifty-six samples from 9 populations of R. mucronata in the Indo-West Pacific region were genotyped; all loci were polymorphic, with the number of alleles ranging from 2 to 9. The mean expected heterozygosity per locus was 0.16 in a population from Sabah, no significant linkage disequilibrium was found among loci, and significant deviation from Hardy?CWeinberg equilibrium was found in 3 loci. The polymorphic microsatellite markers with samples covering most of the species?? distribution range can be applied in genetic diversity studies covering a broad geographical range of the species. |
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