Isolation and characterization of microsatellite loci in Jinshaia sinensis |
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Authors: | Ke Shao Fang Shi Huiyuan Tang Meihua Xiong Weitao Li Bin Zhu |
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Affiliation: | 1. Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan, 430079, People??s Republic of China
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Abstract: | In this study, we isolated and characterized thirteen polymorphic microsatellite markers for Jinshaia sinensis, a fish species endemic to the upper reaches of the Yangtze River in China. Each locus was screened in a population of 48 individuals. Number of alleles per locus ranged between five and nineteen. Observed heterozygosity ranged between 0.121 and 0.854, and expected heterozygosity between 0.722 and 0.928. No significant linkage disequilibrium was found between pairs of loci. However, three loci showed significant departures from Hardy-Weinberg equilibrium and four loci had evidence of null alleles. These markers presented here will be valuable tools to understand the genetic structure of J. sinensis populations in the Yangtze River. |
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