Isolation and characterization of 15 microsatellite loci in Acrossocheilus labiatus Regan (Cyprinidae) |
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Authors: | Lina Chen Zhongbao Li Xiaojiao Chen Yuanyu Cao Qinghui Li Zhihong Zhang Xinjiang Tian |
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Affiliation: | 1. Fisheries College, Jimei University, Xiamen, 361021, China
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Abstract: | Acrossocheilus labiatus Regan occurs in the clear mountain streams in the east of Asia. Eighteen microsatellite loci were isolated from A. labiatus and fifteen loci were polymorphic observed in 30 samples tested. The number of alleles per locus ranged from 2 to 10. The polymorphism information content varied from 0.090 to 0.825. The observed and expected heterozygosities ranged from 0.069 to 0.900 and from 0.067 to 0.844, respectively. Although significant deviations from Hardy?CWeinberg equilibrium were detected for three loci (HC-G11-1, HC-C3-3 and HC-G8-1, P?0.01), no significant deviations were detected for other twelve loci (P?>?0.05). These microsatellite loci will be useful for the further study of population genetic analysis and subsequently provide important genetic data for the conservation and recovery of the A. labiatus resources. |
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