大鼠单侧睾丸损伤对对侧睾丸组织的影响及其机制的探讨

目的：研究大鼠单侧睾丸损伤后对侧睾丸组织变化及其发生机制。方法：选择SD雄性大鼠40只，随机分为4组：A组为对照组，B组为单侧睾丸挫伤组，c组为单侧睾丸裂伤组，D组为裂伤后腹腔注射环孢素A（cyclosporinA，CsA）组。分别于第2、4周观察大鼠后足垫反应（footpadreaction，FPR），取对侧睾丸做苏木精一伊红（HE）染色和免疫组化检测CD4分子、CD8分子的表达。结果：注射睾丸抗原48h后，B、C组大鼠右足足垫较A、D组显著增厚（P〈0．01），C组与B组差异亦有显著统计学意义（P〈0．05），A组与D组差异无统计学意义（P〉0．05）。B、C组对侧睾丸组织学发生明显改变，A、D组改变不明显。B、C组中CU’T细胞平均光密度（oD）值高于CD。’T细胞（P〈0．05），两种T细胞OD值均明显高于对照组（P〈0．01），C组与B组比较，CD4＋T细胞OD值差异有统计学意义（P〈O．05），CD4＋T细胞OD值差异无统计学意义（P〉0．05）。各组结果在第2、4周比较，差异均无统计学意义。结论：单侧睾丸损伤可引起对侧睾丸组织发生迟发型超敏反应（delayedtypehypersensitivity，DTH），主要由CD4＋T细胞介导，且与睾丸损伤程度有关。CsA可抑制DTH的发生。

The Effect on Contralateral Testicular and Its Mechanism After Unilateral Testicular Trauma in Rats

Objective：To study the changes of contralateral testicular histology and its mechanism after Unilateral testicular trauma in rats. Methods： Forty male SD rats were randomly divided into groups A （control）, B（unilateral testieular contusion）, C（unilateral testicular rupture） and D（cyclosporine A was injected into abdominal cavity after testicular rupture）. Foot pad reaction was detected two weeks and four weeks after testicular trauma, contralateral testis was taken for hematoxylin and eosin stain ,expression of CD4 and CD8 was performed by immunohistochemistry. Results：Compare to A, D group,the right foot pad thickness of B, Cgroup was increased greatly forty--eight hours after injection of the test antigens（P~ 0.01 ）. There were significant histological changes in the contralateral testis. B, C group, CD4＋ T lymphocyte in the average optical density（OD） value was higher than CD4＋ T lymphocyte （P〈0.05）. Both of the kinds of T lymphocyte in the average optical density（OD） value was higher than the control group （P〈0.01）. The results of each group in the first two weeks, four weeks showed no significant difference. Conclusions：Delayed type hypersensitivity occurs in contralateral testis after unilateral testic- ular trauma in rats, mainly mediated by CD4 ＋ T cell, which depends on the degree of testicular injury. Cyclosporin A can inhibit the occurrence of delayed type hypersensitivity.