| RP-HPLC法测定陕西产茜草中QA的含量 |
| |
| 引用本文： | 贺光东,郅妙利,王二辉,康文艺. RP-HPLC法测定陕西产茜草中QA的含量[J]. 河南大学学报(医学版), 2008, 27(3) |
| |
| 作者姓名： | 贺光东 郅妙利 王二辉 康文艺 |
| |
| 作者单位： | 河南大学,天然药物研究所,河南,开封,475004;河南大学,药学院,河南,开封,475004;河南大学,天然药物研究所,河南,开封,475004;河南大学,药学院,河南,开封,475004;河南大学,天然药物研究所,河南,开封,475004;河南大学,药学院,河南,开封,475004;河南大学,天然药物研究所,河南,开封,475004;河南大学,药学院,河南,开封,475004 |
| |
| 基金项目： | 河南省科技厅基础前沿计划，河南省教育厅基础研究计划，河南大学校科研和教改项目 |
| |
| 摘 要： | 目的:测定陕西产茜草中1,3,6-trihydroxy-2-methylanthraquinone-3-O-3-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]的含量。方法:选用Purospher star RP-C18色谱柱(4.6 mm×250 mm,5μm,Merck公司);流动相为甲醇-水-四氢呋喃(65∶34.7∶0.3),流速为1.0 ml/min,检测波长为276 nm,柱温为25℃,外标法定量分析。结果:该方法在0.020μg~0.160μg范围内线性关系良好,r=0.9999,平均回收率101.4,RSD=2.0%(n=6),测定结果为0.053%。结论:该法测定茜草中1,3,6-trihydroxy-2-methylanthraquinone-3-O-3-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]含量灵敏、准确、重现性好。
|
| 关 键 词： | HPLC QA 茜草根 |
Determination of the QA contents in Rubia.cordifolia from Shanxi Province by RP-HPLC |
| |
| HE Guang-dong,LI Miao-li,WANG Er-hui,KANG Wen-yi. Determination of the QA contents in Rubia.cordifolia from Shanxi Province by RP-HPLC[J]. Journal of Henan University, 2008, 27(3) |
| |
| Authors: | HE Guang-dong LI Miao-li WANG Er-hui KANG Wen-yi |
| |
| Affiliation: | HE Guang-dong1,2,ZHI Miao-li1,WANG Er-hui1,KANG Wen-yi1,2*(1.Institute of Natural Products,Henan University,Kaifeng 475004,China,2.Pharmaceutical College of Henan University,China) |
| |
| Abstract: | Objective: To determine 1,3,6-trihydroxy-2-methylanthraquinone-3-O-3-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside] in Rubia.cordifolia from Shanxi Province by RP-HPLC.Methods: The HPLC system consisted of Purospher star RP-C18 column(4.6 mm × 250 mm,5 μm,Merck) at 25 ℃,MeOH-H2O-tetrahydropyrane(65∶ 34.7∶ 0.3) mixture as mobile phase,detection wavelength at 276 nm,flow rate of 1.0 ml/min.Results: The linearity was found in the range of 0.020-0.160 μg,r=0.9999,the average recovery was 101.4%,RSD was 2.0%(n=6).The contents of 1,3,6-trihydroxy-2-methylanthraquinone-3-O-3-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside] in Rubia.cordifolia from Shanxi province was 0.053%.Conclusion: The method is reliable and suitable for determination of contents of 1,3,6-trihydroxy-2-methylanthraquinone-3-O-3-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside] in Rubia.cordifolia from Shanxi Province. |
| |
| Keywords: | HPLC QA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
