| 硫化砷对K_(562)/ADM细胞HSP70蛋白表达的影响 |
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| 引用本文： | 张晨,黄世林,向阳,白月辉. 硫化砷对K_(562)/ADM细胞HSP70蛋白表达的影响[J]. 中国癌症杂志, 2001, 0(1) |
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| 作者姓名： | 张晨 黄世林 向阳 白月辉 |
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| 作者单位： | 解放军第210医院中医血液病研究所!辽宁大连116021 |
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| 摘 要： | 目的 :探讨硫化砷 (As2 S2 )对K5 6 2 及其耐药细胞株K5 6 2 ADM细胞膜HSP70蛋白表达的影响。方法 :采用流式细胞仪 (FCM)测定K5 6 2 和耐药细胞K5 6 2 ADM细胞膜HSP70蛋白表达及细胞凋亡 ,利用RT PCR方法检测HSP70的mRNA水平。结果 :硫化砷能增加细胞膜HSP70蛋白表达 ,且与时间及浓度成正相关 ,而在mRNA水平上仍有较高的表达。K5 6 2 ADM细胞膜HSP70蛋白表达和细胞凋亡率均高于K5 6 2 细胞。结论 :硫化砷能增加细胞膜HSP70蛋白表达 ,且这种改变可能对细胞凋亡率产生一定影响
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| 关 键 词： | 砷制剂 HSP70 K_(562)/ADM 凋亡 |
Influence of arsenic bisulfide on the expression of HSP70 in K_(562)/ADM cell |
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| ZHANG Chen,HUANG Shi lin,XIANG Yang,et al. Influence of arsenic bisulfide on the expression of HSP70 in K_(562)/ADM cell[J]. China Oncology, 2001, 0(1) |
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| Authors: | ZHANG Chen HUANG Shi lin XIANG Yang et al |
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| Abstract: | Purpose:To study the expression of membrane protein HSP70 of K 562 /ADM cell using arsenic bisulfide(As 2S 2) treatment Methods:Membrane HSP70 and apoptosis of K 562 and multidrug resistant K 562 /ADM cell were studied by flow cytometry assay and RT PCR Results:Membrane HSP70 of K 562 and K 562 /ADM cell gradually increased from 12 to 24 hours with arsenic bisulfide treatment, and had higher expression at the level of HSP70mRNA Expression of membrane HSP70 and apoptosis of K 562 /ADM was higher than that of K 562 Conclusions:Arsenic bisulfide treatment can increase expression of membrane HSP70 of K 562 /ADM and K 562 cells.The change of membrane HSP70 is probably related to K 562 /ADM cell apoptosis |
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| Keywords: | arsenical agents HSP70 K 562 /ADM apoptosis |
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