Orphanin FQ/nociceptin binds to functionally coupled ORL1 receptors on human immune cell lines and alters peripheral blood mononuclear cell proliferation |
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Authors: | Peluso J Gavériaux-Ruff C Matthes H W Filliol D Kieffer B L |
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Affiliation: | CNRS UPR9050, ESBS and Faculty of Pharmacy, Illkirch, France. |
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Abstract: | Orphanin FQ/nociceptin (OFQ/N) has been shown to modulate nociception, responses to stress and anxiety. We investigated OFQ/N function in human immune cells. We find that monocytic U937, T lymphocytic CEM, and MOLT-4 cell lines express OFQ/N binding sites at levels comparable to that of human SH-SY5Y neuroblastoma cells. We show that OFQ/N receptors are functionally coupled to G proteins in these cells. Finally OFQ/N decreases proliferation of phytohemagglutinin-stimulated peripheral blood mononuclear cells in vitro at doses ranging from 10(-13) to 10(-8) M. Thus, our data suggest that OFQ/N and OFQ/N receptor may act as an immunomodulatory system. |
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Keywords: | Orphanin FQ/nociceptin ORL-1 receptor Immune cells Proliferation Human |
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