大豆异黄酮对糖尿病大鼠心肌损伤保护的实验研究

目的:探讨大豆异黄酮(soy isoflavones,SI)对糖尿病大鼠心肌损伤的保护作用。方法:SD大鼠48只,随机分为正常对照组(N C)、糖尿病对照组(D C)、低剂量SI治疗组(L-SI)、中剂量SI治疗组(M-SI)、高剂量SI治疗组(H-SI)、尼尔雌醇治疗组(N T)。后5组腹腔注射链脲佐菌素55m g/kg制备D M模型。第7周起,N C、D C组予0.5%C M C-N a10m L/(kg·d),L-SI、M-SI、H-SI组分别予大豆异黄酮30、60、120m g/(kg·d),N T组予尼尔雌醇混悬液0.1m g/kg(2次/周)灌胃。第12周末测大鼠心肌组织N a+-K+-A TPase、C a2+-A TPase活性、N O S活性和N O含量。电镜观察超微结构变化。结果:D C组心肌组织N a+-K+-A TPase、C a2+-A TPase、N O S活性和N O含量明显降低(P<0.01),肌小节和线粒体形态改变。而M-SI、H-SI可抑制上述变化。结论:中、高剂量SI对糖尿病大鼠心肌损伤具有保护作用。

Protective effect of soy isoflavones on myocardial injury in diabetic rats

Objective To investigate the effect of soy isoflavones on myocardial injury in diabetic rats. Methods Forty eight SD rats were randomly divided into six groups:normal control group (NC),diabetic control group (DC),three SI treatment groups (L SI,M SI,H SI) and nilestrioli treatment group (NT). Rats were given streptozotocin (STZ) 55 mg/kg intraperitneally (ip) except NC group rats. After 6 weeks,SI 30,60,120 mg/(kg·d) were given in L SI,M SI,H SI groups,nilestriol 0.2 mg/(kg·wk) was given in NT group,and th...