血管内皮生长因子在子宫内膜异位症发生中的作用探讨

目的：探讨血管内皮生长因子（VEGF）在子宫内膜异位症（EM）发生发展中的作用。方法：采用酶联免疫吸附法（ELISA）测定EM组及对照组腹腔液及血清VEGF含量,采用免疫组织化学SP法检测异位内膜、在位内膜和正常子宫内膜组织中VEGF的表达。结果：EM组腹腔液及血清VEGF含量明显高于对照组,2组比较有极显著性差异（P〈0.01）;VEGF在异位内膜组、在位内膜组和对照组均有表达,阳性表达率分别为63.33%、46.00%、23.33%,表达强度依次减弱,3组阳性构成比差异有统计学意义,3组中任何2组之间差异均有统计学意义（P〈0.01）。异位内膜组和在位内膜组VEGF表达分泌期强于增生期,差异有显著性（P〈0.05）;对照组增生期和分泌期表达强度均较弱,差异无显著性意义（P〉0.05）。结论：VEGF在EM发生发展中发挥重要作用。

Study on the Role of VEGF in Development of Endometriosis

Objective To study the role of vascular endothelial growth factor（VEGF）in the development of endometriosis.Methods VEGF levels in peritoneal fluid and serum of the endometriosis group and control group were determined by ELISA.VEGF expressions in ectopic endometrium,eutopic endometrium and normal endometrium were detected by immunohistochemical SP method.Results IVEGF levels in peritoneal fluid and serum in endometriosis group were significantly higher than those in the control group（P〈0.01）;TheVEGF positive expression rates in ectopic endometrium,eutopic endometrium and normal endometrium were 63.33%,46.00%, 23.33%,respectively.The difference of positive expression rates showed statistically significant between any two groups of the three groups（P〈0.01）.VEGFexpressionsinsecretory phase of ectopic endometrium and eutopic endometrial cells were stronger than in proliferative phase（P〈0.05）;In control group,in proliferative and secretory phase the expression were weaker,the difference have no statistically significant（P〉0.05）.Conclusion VEGF plays an important role in the development of endometriosis.