错配修复基因 hMLH1 和hMSH2 在胃癌组织中的表达及意义

目的探讨错配修复基因hMLH1 和hMSH2 在胃癌发生中的作用.方法采用免疫组化SP法,检测散发性胃癌、癌旁黏膜和胃炎黏膜组织中hMLH1 和 hMSH2基因的表达情况.结果 hMSH2在胃癌组织中的阳性表达率(67.1%)显著高于癌旁黏膜(41.4%)和胃炎黏膜组织(41.2%)(P<0.05),而后两者无显著差异(P>0.05).hMSH2在低分化腺癌中的阳性率(80.6% )显著高于高中分化腺癌(57.7%)和黏液癌(53.8%)(P<0.05),而后两者无显著差异(P>0.05).hMLH1在胃炎黏膜组织的阳性表达率(38.2%)显著低于胃癌(77.1%)和癌旁黏膜组织(74.3%)(P<0.05),后两者无显著差异(P>0.05). hMLH1在黏液癌中的阳性率(38.5%)显著低于高中分化腺癌(80.8%)和低分化腺癌(90.3%)(P<0.05),后两者无显著差异(P>0.05).结论 hMSH2高表达可能是胃癌发生的标志之一;hMSH2表达与肿瘤分化程度及患者预后有关;hMLH1有可能作为胃癌预警组织学标记物.

Mismatch repair gene hMLH1 and hMSH2 expression in gastric cancer tissue and its significance

Objective To investigate the role of mismatch repair gene hMLH1 and hMSH2 in carcinogenesis of gastric cancer. Methods The expression of hMLH1 and hMSH2 in sporadic gastric cancer, adjacent mucosa and gastritis mucosa tissue was examined by immunohistochemistry SP method. Results The positive rate of hMSH2 expression in cancer tissue (67.1%) was significantly higher than that in adjacent mucosa (41.4%) and gastritis mucosa tissue (41.2%)(P<0.05); the positive rate in poor differentiated group (80.6%) was significantly higher than those in well differentiated group (57.7%) and mucoid cancer group (53.8%)(P<0.05), but there was no significant difference between the latter two groups (P>0.05). The positive rate of hMLH1 expression (38.2%) was significantly lower than that in gastric cancer (77.1%) and adjacent mucosa tissue (74.3%)(P<0.05); the positive rate in mucoid cancer (38.5%) was significantly lower than those in well (80.8%) and poor differentiated group (90.3%)(P<0.05), but there was no significant difference between the latter two groups (P>0.05). Conclusions The high expression of hMSH2 may be one of potential markers of gastric cancer; the expression of hMSH2 is related to tumor differential degree and prognosis; hMLH1 protein may be a histological marker predicting gastric cancer occurrence.