| 叶酸对体外人胃腺癌细胞SGC-7901生长的作用 |
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| 引用本文： | 杨小珊,曾令福. 叶酸对体外人胃腺癌细胞SGC-7901生长的作用[J]. 中国公共卫生, 2003, 19(12): 1445-1446 |
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| 作者姓名： | 杨小珊 曾令福 |
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| 作者单位： | 1. 重庆医科大学预防医学系营养与食品卫生学教研室,重庆,400016
2. 四川大学华西公共卫生学院营养与食品卫生学教研室 |
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| 摘 要： | 目的 以体外培养的人胃癌细胞株SGC—7901为材料，观察叶酸的抑癌防癌作用。方法 采用生长曲线法、MTT法观察叶酸对细胞的增殖和功能的影响；采用FCM进行细胞周期分析，观察叶酸对细胞凋亡的影响。结果 在叶酸浓度为375，750和1875nmol／L时对人胃癌细胞株的增殖和功能有抑制作用，浓度为375nmol／L时引起的凋亡率可达33．1％。结论 叶酸对人胚肌腱细胞无抑制作用，表明叶酸对正常人体细胞无不良影响而对癌细胞有抑制作用。
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| 关 键 词： | 胃癌细胞株 叶酸 抑癌 细胞凋亡 |
| 文章编号： | 1001-0580(2003)12-1445-02 |
| 修稿时间： | 2003-04-15 |
Effects of folic acid on growth of human gastric cancer cell line in vitro |
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| YANG Xiao-shan,ZENG Ling-fu.Preventive Medicine Departme nt,Chongqing University of Medical Sciences. Effects of folic acid on growth of human gastric cancer cell line in vitro[J]. Chinese Journal of Public Health, 2003, 19(12): 1445-1446 |
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| Authors: | YANG Xiao-shan ZENG Ling-fu.Preventive Medicine Departme nt Chongqing University of Medical Sciences |
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| Abstract: | ObjectiveTo study the antitumor activities of folic acid .Folic acid are dealed with human gastric cancer cell line SGC-7901 in vitro. MethodsBy means of four approaches as follows:cell prol iferation curve analysis,MTT assay and cell cycle analysis by FCM.ResultsAfter treatment with 375,750 and 1?875?nmol/L,f olic acid selectively inhibited the activity and function of SGC-7901.There are apoptotic peaks in cell cycle analysis by FCM and apoptosis rate reached 33.1 % .ConclusionThe effects of folic acid on human embryo musc le sinew cells were slightly,which indicated that folic acid had no bad effects on normal cell,but inhibited tumor cell. |
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| Keywords: | gastric cancer cell line folic acid antitumor cell apoptosis |
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